文献类型：期刊文献

英文题名：TBHQ improved neurological recovery after traumatic brain injury by inhibiting the overactivation of astrocytes

作者：Zhang, Zhen-Wen[1,2];Liang, Jun[3];Yan, Jing-Xing[2,3];Ye, Yi-Chao[3];Wang, Jing-Jing[3];Chen, Chong[3];Sun, Hong-Tao[3];Chen, Feng[2];Tu, Yue[3];Li, Xiao-Hong[4]

第一作者：张震文

通信作者：Tu, Y[1];Li, XH[2]

机构：[1]Gansu Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China;[3]Characterist Med Ctr PAPF, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Tianjin 300162, Peoples R China;[4]Tianjin Univ, Acad Med Engn & Translat Med, Tianjin 300072, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Characterist Med Ctr PAPF, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Tianjin 300162, Peoples R China;[2]corresponding author), Tianjin Univ, Acad Med Engn & Translat Med, Tianjin 300072, Peoples R China.

年份：2020

卷号：1739

外文期刊名：BRAIN RESEARCH

收录：;Scopus(收录号：2-s2.0-85083310330);WOS:【SCI-EXPANDED(收录号:WOS:000532820500005)】；

基金：This work was supported by the National Nature Scientific Fund of China (81771350, 81771352, 81671222, 81772018, 81971782) and the Nature Scientific Fund of Tianjin (18JCJQJC48500, 15ZXLCSY00040, 16JCYBJC27600, 16ZXHLSY00120).

语种：英文

外文关键词：Traumatic brain injury; TBHQ; Astrocyte; Activation; Nrf-2; Oxidative stress

摘要：Traumatic brain injury (TBI) is a major leading cause of death and long-term disability. Although astrocytes play a key role in neuroprotection after TBI in the early stage, the overactivation of astrocytes can lead to long-term functional deficits, and the underlying pathophysiological mechanisms remain unclear. In addition, it is unknown whether the nuclear factor erythroid 2-related factor2/haem oxygenase-1 (Nrf-2/HO-1) pathway could elicit a neuroprotective effect by decreasing astrocyte overactivation after TBI. We aimed to study the effects of tert-butylhydroquinone (TBHQ) in reducing astrocyte overactivation after TBI and explored the underlying mechanisms. We first established a controlled cortical impact (CCI) model in rats and performed Haematoxylin and eosin (H&E) staining to observe brain tissue damage. The cognitive function of rats was assessed by modified neurological severity scoring (mNSS) and Morris water maze (MWM) test. Astrocyte and microglia activation was detected by immunofluorescence staining. Oxidative stress conditions were investigated using Western blotting. An enzyme-linked immunosorbent assay (ELISA) was designed to assess the level of the proinflammatory factor tumour necrosis factor-alpha (TNF-alpha). Dihydroethidium (DHE) staining was used to detect reactive oxygen species (ROS). Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The results showed that the administration of TBHQ ameliorated motor function and cognitive deficits and decreased the lesion volume. In addition, TBHQ significantly decreased astrocyte overactivation, diminished the pro-inflammatory phenotype M1 and inflammatory cytokines production after TBI, increased Nrf-2 nuclear accumulation, and enhanced the levels of the Nrf-2 downstream antioxidative genes HO-1 and NADPH-quinone oxidoreductase-1 (NQO-1). Furthermore, TBHQ treatment alleviated apoptosis and neuronal death in the cerebral cortex. Overall, our data indicated that the upregulation of Nrf-2 expression could enhance neuroprotection and decrease astrocyte overactivation and might represent a new theoretical basis for treating TBI.