文献类型：期刊文献

中文题名：大黄牡丹汤对急性胰腺炎大鼠肝损伤的保护作用

英文题名：Protective effect of Dahuang Mudan Decoction on liver injury in rats with acute pancreatitis

作者：孙银凤[1,2];杨丹[1];白敏[1];宋冰[1,2];康万荣[1,2];汪永锋[1,2];张延英[1,2]

第一作者：孙银凤

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃中医药大学甘肃省实验动物行业技术中心,兰州730000

第一机构：甘肃中医药大学

年份：2022

卷号：30

期号：2

起止页码：169

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金(82160871,81760828);甘肃中医药大学研究生创新基金(2021CX35);甘肃中医药大学科学研究与创新基金项目(2021KCYB-6)。

语种：中文

中文关键词：急性胰腺炎;大黄牡丹汤;肝损伤;PI3K/Akt/NF-κB信号通路

外文关键词：acute pancreatitis;Dahuang Mudan Decoction;liver injury;PI3K/Akt/NF-κB signaling pathway

摘要：目的探讨大黄牡丹汤对急性胰腺炎大鼠肝损伤的保护作用。方法将96只SPF级Wistar大鼠随机分为空白组、模型组、大黄牡丹汤高、中、低剂量组(14、7、3.5 g/(kg·d))、奥曲肽组(10μg/(kg·d)),各16只,采用5%牛磺胆酸钠溶液逆行注射胰胆管复制模型,于造模前1 h及造模后12、24 h分别给药1次,采集样本。观察大鼠一般体征,生化测定血清淀粉酶(AMS)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、胆碱酯酶(ChE)、C反应蛋白(CRP)含量,HE染色观察胰腺和肝组织病理形态变化,ELISA法检测大鼠肝组织TNF-α、IL-1β、IL-6含量;Western Blot法检测大鼠肝组织PI3K/Akt/NF-κB通路关键蛋白表达水平。结果(1)与空白组比较,模型组大鼠一般生存状况相对较差,AMS、ALT、AST、CRP含量增高,ChE含量降低;HE染色镜下可见胰腺组织结构不完整、坏死、充血和炎性浸润严重,肝细胞排列紊乱、坏死、脂肪样变、炎症浸润明显;肝组织匀浆液中TNF-α、IL-1β、IL-6含量均显著升高;肝组织中PI3K、p-Akt、p-NF-κB p65、Bax蛋白表达水平均升高,Bcl-2蛋白表达水平下降(P<0.05)。(2)与模型组比较,各治疗组大鼠生存状态改善;镜下胰腺组织水肿、充血、坏死程度明显改善,肝细胞排列紊乱、坏死、脂肪样变、炎症浸润等情况明显减轻;AMS、ALT、AST、CRP含量不同程度降低,ChE含量升高;肝组织匀浆液中TNF-α、IL-1β、IL-6含量均下降;肝组织中PI3K、p-Akt、p-NF-κB p65、Bax蛋白表达水平均下降,Bcl-2蛋白表达水平升高(P<0.05)。结论大黄牡丹汤有效改善急性胰腺炎(acute pancreatitis,AP)模型大鼠一般状态、生化指标以及胰腺和肝组织病理改变,其作用机制可能是通过调控PI3K/Akt/NF-κB信号通路,从而减轻肝损伤。
Objective To investigate the protective effects of Dahuang Mudan against liver injury in rats with acute pancreatitis.Methods Overall,96 SPF Wistar rats were randomly divided into blank,model,Dahuang Mudan decoction high-,medium-,and low-dose(14,7,3.5 g/(kg·d),respectively),and octreotide(10μg/(kg·d))groups,with 16 animals in each group.The acute pancreatitis model was generated by retrograde injection of 5%sodium taurocholate solution into the pancreaticobiliary duct.before the model and every 12 h after the model.Samples were collected 24 h after the mold.To observe the rats’general physical signs,serum amylase(AMS),alanine aminotransferase(ALT),aspartate aminotransferase(AST),cholinesterase(ChE),and C-reactive protein(CRP)contents were biochemically determined,and hematoxylin and eosin staining used to observe pancreatic and hepatic histopathological changes.ELISA was employed to detect rat liver tissue TNF-α,IL-1β,and IL-6 contents,and western blotting was used to detect the expression of key rat liver PI3 K/Akt pathway proteins.Results(1)Compared with the blank group,the model group rats’general survival status was relatively poor;their contents of AMS,ALT,AST,and CRP increased and that of ChE decreased.Microscopic analysis of HE staining showed the pancreatic tissue had incomplete structure,and was necrotic and congested with severe inflammatory infiltration;liver cells showed disordered arrangement,and liver tissue showed necrosis,steatosis,and inflammatory infiltration.The TNF-α,IL-1β,and IL-6 contents of the liver tissue homogenate were significantly increased;PI3 K,p-Akt,p-NF-κB p65,and Bax protein expression levels decreased and Bcl-2 protein expression increased,especially in the Dahuang Mudan high-dose group,and the difference was statistically significant(P<0.05).(2)Compared with the model group,the general condition of the rats in each treatment group improved to varying degrees:the degree of pancreatic tissue edema,congestion,and necrosis visible under the microscope was significantly improved,and liver cell arrangement disorder,necrosis,steatosis,inflammatory infiltration,among others,were alleviated.AMS,ALT,AST,and CRP contents decreased to varying degrees,and the ChE content increased.Liver tissue homogenate TNF-α,IL-1β,and IL-6 contents decreased;PI3 K,p-Akt,p-NF-κB p65,and Bax protein levels decreased;while the levels of Bcl-2 protein increased,especially in the Dahuang Mudan high-dose group,and the differences were statistically significant(P<0.05).Conclusions Dahuang Mudan decoction effectively improved the general health,biochemical indicators,and pathological changes to the pancreas and liver of acute pancreatitis model rats.The mechanism of its liver-damage-reducing action may involve regulation of the PI3 K/Akt signaling pathway.