文献类型：期刊文献

中文题名：西黄丸对乳腺增生大鼠乳腺组织形态和免疫功能的影响

英文题名：Effect of Xihuang Pills on Mammary Tissue Morphology and Immune Function of Hyperplasia of Mammary Glands in Rats

作者：连小龙[1];赵敏[1];韩宇[2];王秋兰[3];韩涛[1]

第一作者：连小龙

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]华中科技大学生命科学与技术学院,湖北武汉430074;[3]甘肃中医药大学临床医学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：33

期号：6

起止页码：496

中文期刊名：解放军药学学报

外文期刊名：Pharmaceutical Journal of Chinese People's Liberation Army

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然基金项目;No.81560722

语种：中文

中文关键词：西黄丸;乳腺增生;免疫功能;大鼠

外文关键词：Xihuang pills ; hyperplasia of mammary glands ; immune function ; rats

摘要：目的探讨西黄丸对乳腺增生大鼠乳腺组织形态和免疫功能的影响。方法 60只大鼠随机均分为正常组、模型组、他莫昔芬组(0.0018g·kg^(-1))和西黄丸低、中、高剂量组(0.54、1.08、2.16g·kg^(-1))。除正常组外,各组大鼠均肌肉注射苯甲酸雌二醇和黄体酮以诱导乳腺增生模型,复制模型同时给药组灌胃相应药物,正常组和模型组灌胃等体积蒸馏水。游标卡尺测量大鼠乳头直径和高度;Elisa试剂盒测定大鼠血清中雌二醇、孕酮和白细胞介素-2、肿瘤坏死因子-α水平,计算脏器指数(胸腺、脾脏),HE染色并观察大鼠乳腺组织形态学变化,采用立体定量分析方法观察乳腺小叶体密度、间质体密度和腺泡比表面。结果西黄丸低、中、高剂量可显著升高乳腺增生大鼠血清中白细胞介素-2、孕酮水平(P<0.05,P<0.01),降低肿瘤坏死因子-α、雌二醇水平(P<0.05,P<0.01);大鼠胸腺、脾脏指数显著增大(P<0.05,P<0.01);乳腺小叶体密度、腺泡比表面降低(P<0.05,P<0.01),小叶间质体密度增加(P<0.05,P<0.01)。结论西黄丸能够抑制大鼠乳腺增生,其作用机制可能与调节激素水平、增强免疫清除功能相关。
Objective To explore the effect of Xihuangpills on mammary tissue morphology and immune function of rats with mammary gland hyperplasia.Methods Sixty rats were randomly divided into normal group,model group,tamoxifen group（0.0018 g·kg^-1）and Xihuangpill low,middle and high dose groups（0.54,1.08,2.16 g·kg^-1）.The model of mammary gland hyperplasia was induced by estradiol benzoate and progesterone in each group except the normal group.The replicated models were administered with corresponding drugs,while the normal group and model group were given distilled water.The levels of Estradiol（E2）,Progesterone（P）and IL-2,TNF-αin the serum of rats were measured with Elisa kit.The organ indexes（thymus,spleen）and HE staining were calculated,the morphological changes of the mammary gland were observed,and the density of breast lobules,interstitial density and the acinar surface were observed with stereotaxic analysis.Results Xihuangpills low,middle and high dose obviously increased the levels of IL-2 and P（P〈0.05,P〈0.01）,decreased the levels of TNF-αand E2（P〈0.05,P〈0.01）in serum of rats with mammary gland hyperplasia,and improved the indexes of the thymus and spleen（P〈0.05,P〈0.01）.Pathological morphology showed that the density of breast lobules and the acinar surface of acinar were significantly decreased（P〈0.05,P〈0.01）,while the interstitial density was increased distinctly（P〈0.05,P〈0.01）.Conclusion Xihuangpills can inhibit mammary gland hyperplasia in rats,its mechanism may be related to the regulation of hormone levels and enhance immune dearance.