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sCD14在痛风性关节炎患者炎症反应中的变化及其意义     被引量:14

The changes and significance of sCD14 in gouty arthritis patients'inflammatory reaction

文献类型:期刊文献

中文题名:sCD14在痛风性关节炎患者炎症反应中的变化及其意义

英文题名:The changes and significance of sCD14 in gouty arthritis patients'inflammatory reaction

作者:党万太[1,2];王婧[3];谢文光[1];周京国[1]

第一作者:党万太

机构:[1]川北医学院附属医院风湿免疫研究所,四川南充637000;[2]成都中医药大学临床医学院,四川成都610075;[3]甘肃中医学院,甘肃兰州733005

第一机构:川北医学院附属医院风湿免疫研究所,四川南充637000

年份:2015

卷号:36

期号:4

起止页码:514

中文期刊名:西安交通大学学报(医学版)

外文期刊名:Journal of Xi’an Jiaotong University(Medical Sciences)

收录:CSTPCD;;北大核心:【北大核心2014】;CSCD:【CSCD_E2015_2016】;

基金:国家自然科学基金资助项目(No.81272047);四川省科技创新苗子工程资助项目(No.2014-087)~~

语种:中文

中文关键词:痛风性关节炎;sCD14;炎症;白介素;1β(IL-1β);肿瘤坏死因子α(TNF-α);C;反应蛋白(CRP)

外文关键词:gouty arthritis;inflammation soluble membrane CD14 (sCD14) interleukin 1β(IL-1β) tumor necrosis factor-α(TNF-α) C reaction protein (CRP)

摘要:目的探讨可溶性白细胞分化抗原14(sCD14)在痛风性关节炎(GA)患者炎症反应中的变化及其意义。方法选取31例急性GA(AGA)患者、23例非AGA(NAGA)患者和20名健康对照者(HC),用实时荧光定量聚合酶链反应(qRT-PCR)检测其外周血单个核细胞(PBMCs)中CD14的mRNA表达水平;用酶联免疫吸附法(ELISA)检测各组血浆sCD14、白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)蛋白表达水平,用免疫比浊法测定各组血浆C反应蛋白(CRP)含量;检测各组白细胞计数(WBC)、嗜中性粒细胞绝对值(GR)、淋巴细胞绝对值(LY)、单核细胞绝对值(MO)等血常规及血尿酸(UA)等指标变化,比较3组指标的差异并分析其与PBMCs的CD14mRNA表达及血浆sCD14表达的相关性。结果 AGA组PBMCs中CD14mRNA表达量高于HC组(P<0.05);HC与NAGA组血浆sCD14蛋白表达低于AGA组(P<0.01),且NAGA组表达低于HC组(P<0.05);AGA组CRP蛋白水平高于HC与NAGA组(P<0.01);AGA、NAGA组血浆IL-1β、TNF-α蛋白水平均显著高于HC组(P<0.01),且AGA组IL-1β蛋白水平高于NAGA组(P<0.01);AGA组和NAGA组WBC高于HC组(分别P<0.01,P<0.05);AGA组GR、MO均高于HC组(P<0.05),AGA组MO高于NAGA组(P<0.05);AGA与NAGA组UA均显著高于HC组(P<0.01)。AGA组PBMCs的CD14mRNA表达与IL-1!呈正相关(rs=0.362,P=0.045);NAGA组血浆sCD14蛋白表达与GR呈正相关(rs=0.397,P=0.030)。结论 sCD14在GA患者的炎症反应中表达异常,提示sCD14在GA患者的炎症反应中可能发挥重要的调控作用。
Objective To study the changes and significance of sCD14 in inflammatory response of patients with gouty arthritis.Methods CD14 mRNA was measured using quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs).The expression of CD14 mRNA in PBMCs was compared between patients with acute gouty arthritis (AGA)(n =31)and non-acute gouty arthritis (NAGA)(n =23)and healthy controls (HC)(n =20).β-actin was selected as the internal control.The protein expressions of sCD14,IL-1βand TNF-αwere measured using enzyme linked immunosorbent assay (ELISA) in patients’ plasma.The protein expression of CRP was measured using immunoturbidimetry in patients’ plasma. Routine blood and blood biochemistry indexes were measured by routine blood analyzer and blood biochemistry analyzer of patients with AGA,NAGA and HC.We analyzed the correlation between CD14 mRNA,sCD14 protein expression and each clinical indicator.Results When compared with that in AGA group,the mRNA expression of CD14 increased significantly in PBMCs of HC patients (P 〈 0.05 ).When compared with that in HC and NAGA patients,the protein expression of sCD14 increased significantly in the plasma of AGA patients (P 〈0.01).The protein expression of sCD14 was significantly lower in the plasma of NAGA than in HC (P 〈0.05).The protein expression of sCD14 increased significantly in the plasma of AGA compared with HC and NAGA (P 〈 0.01 ).When compared with those in HC,the protein expressions of IL-1β and TNF-α increased significantly in the plasma of AGA and NAGA (P 〈 0.01 ).When compared with that in NAGA,the protein expression of IL-1βincreased significantly in plasma of AGA (P 〈0.01). The indexes of WBC increased significantly in AGA compared with HC (P 〈0.01),and WBC increased significantly in NAGA compared with HC (P 〈0.05).The indexes of GR and MO increased significantly in AGA compared with HC (P 〈0.05),and MO increased significantly in AGA compared with NAGA (P 〈 0.05 ).The indexes of UA increased significantly in AGA and NAGA compared with HC (P 〈0.01).There was a positive correlation between CD14 mRNA expression and IL-1β in PBMCs in AGA group (r s =0.362,P =0.045).A positive correlation was found between GR and the protein expression of sCD14 in NAGA patients’plasma (r s = 0.397,P = 0.030 ). Conclusion The dysregulated expressions of CD14 mRNA in PBMCs and sCD14 protein in GA show that sCD14 may play a significantly regulatory role in inflammatory reaction.

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