文献类型：期刊文献

中文题名：牦牛AQP9基因CDS全长序列克隆及其生物信息学特征分析

英文题名：Cloning and Bioinformatics Analysis of the CDS of Yak AQP9 Gene

作者：刘健锋[1];丁艳平[2];伍志伟[3];王建林[1];邵宝平[1]

第一作者：刘健锋

机构：[1]兰州大学生命科学学院动物学与发育生物学研究所,兰州730000;[2]西北师范大学生命科学学院,兰州730070;[3]甘肃中医学院系统生物学与中医药转化研究所,兰州730020

第一机构：兰州大学生命科学学院动物学与发育生物学研究所,兰州730000

年份：2015

卷号：46

期号：7

起止页码：1134

中文期刊名：畜牧兽医学报

外文期刊名：ACTA VETERINARIA ET ZOOTECHNICA SINICA

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金项目(青年基金:31000190;地区基金:31060141);兰州大学中央高校基本科研业务费专项

语种：中文

中文关键词：牦牛;AQP9基因;CDS区;分子克隆;生物信息学

外文关键词：yak;Aquaporin9(AQP9);gene;CDS region;molecular cloning;bioinformatics

摘要：为了研究高原动物对青藏高原极端生境的适应机理,并为探讨高原动物适应机制提供基本数据。本研究运用基因克隆技术对牦牛脑AQP9基因CDS全长序列进行克隆,采用生物信息学方法进行分析,AQP9基因和编码序列特征进行了以下几方面的预测和分析:其物化性质、疏水性、跨膜结构和蛋白二级结构。结果表明,牦牛AQP9的CDS含有一个885bp的开放阅读框,编码295个氨基酸;牦牛AQP9基因编码蛋白分子量和理论等电点分别为31.89ku和6.21,其编码蛋白含有6次跨膜结构,属于疏水性蛋白;二级结构由α-螺旋、延伸链、β-折叠及无规则卷曲构成;牦牛AQP9基因编码氨基酸序列与黄牛、藏羚羊、绵羊等物种间同源性较高,系统进化情况与其亲缘关系远近一致。本研究将为牦牛低氧适应性研究提供一定的基础资料。
The aim of this study was to investigate the adaption mechanism of plateau animals to extreme environment of the Qinghai-Tibet plateau,and to provide some basic data for exploring the adaption mechanism of plateau animals.The coding region sequence of yak AQP9 was cloned by molecular cloning technique and analyzed by bioinformatics in this study.Some characteristics of the AQP9 gene and encoded protein sequences were predicted and analyzed in the following aspects as the general physical and chemical properties,hydrophobicity,transmembrane structure and secondary structure.The results showed that the CDS of yak AQP9 contains a complete ORF(885bp)encoding 295 amino acids.The putative molecular weight and theory isoelectric point of AQP9 gene coding protein in yak were 31.89 ku and 6.21,respectively.The protein encoded by yak AQP9 contains 6transmembrane domains,and it was a hydrophobic protein.The secondary structures are mainly composed ofα-helix,extended strand,β-turn and random coil.Deduced amino acid sequences of AQP9 gene between yak and cattle,sheep and other species were high homology,and the phylogenetic distance consistent with their genetic relationship.This study will provide certain basic data for studying hypoxia adaptation of yak.