文献类型：期刊文献

中文题名：抗衰益智胶囊对衰老大鼠脑组织三磷酸腺苷酶活性和血清白细胞介素-6、肿瘤坏死因子-α的影响

英文题名：Effects of Kangshuai Yizhi Capsule on Activity of ATP in Brain Tissues and IL-6,TNF-α in Serum of Aging Rats

作者：郑世铎[1];杨世彰[2];段永强[3];杜娟[3];成映霞[3];张立[3];杨晓轶[3];刘稼[3]

第一作者：郑世铎

机构：[1]甘肃中医学院附属医院;[2]华亭县上关中心卫生院;[3]甘肃中医学院

第一机构：甘肃中医药大学第二附属医院

年份：2014

卷号：21

期号：7

起止页码：40

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省中医药管理局资助项目(W02-1)

语种：中文

中文关键词：抗衰益智胶囊;Na+-K+-ATP酶;Ca2+-Mg2+-ATP酶;白细胞介素-6;肿瘤坏死因子-α;衰老;大鼠

外文关键词：Kangshuai Yizhi Capsule;Na＋-K＋-ATP;Ca2＋-Mg2＋-ATP;IL-6;TNF-α;aging;rats

摘要：目的观察抗衰益智胶囊对衰老模型大鼠脑组织三磷酸腺苷(ATP)酶活性和血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响,探讨其对脑组织的保护作用。方法受试动物按随机数字表法分为空白组和造模组。采用D-半乳糖腹腔注射造模,造模成功的大鼠分为模型组、脑复康组和抗衰益智胶囊高、低剂量组,各给药组给予相应药物灌胃,正常组和模型组每日给予等量生理盐水灌胃,连续60 d,测定各组大鼠脑组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和血清IL-6、TNF-α含量。结果与空白组比较,模型组大鼠脑组织Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性降低,血清IL-6和TNF-α含量增高,差异有统计学意义(P<0.05,P<0.01);与模型组比较,各给药组大鼠脑组织Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性升高,血清IL-6和TNF-α含量降低,差异有统计学意义(P<0.05),且以抗衰益智胶囊高剂量组作用为优。结论抗衰益智胶囊具有提高衰老大鼠脑组织ATP酶活性和降低促炎因子水平的作用。
Objective To observe effects of Kangshuai Yizhi Capsule on ATP in brain tissue and IL-6, TNF-α in serum of aging model rats, and explore the protective effects of the capsule on brain tissue.Methods Totally 72 rats were randomly divided into a normal group and a model group. The subacutely aging model rats were made by injectingD-gal, then aging rats were numbered and grouped by random number table into the model group, Kangshuai Yizhi high-dose group, Kangshuai Yizhi Capsule low-dose group and Naofukang group. All dose groups were received gavage by giving corresponding doses, while normal group and model group were given the same amount of saline everyday. After treated for 60 days, the activity of Na＋-K＋-ATP and Ca2＋-Mg2＋-ATP in brain tissue, and IL-6, TNF-α in serum were detected.Results Compared with normal group, Na＋-K＋-ATP and Ca2＋-Mg2＋-ATP were less active （P〈0.05）, but levels of IL-6 and TNF-α in model group were significantly higher, with statistical significance （P〈0.05）. Compared with model group, after treated with Kangshuai Yizhi Capsule, Na＋-K＋-ATP and Ca2＋-Mg2＋-ATP were more active, and IL-6 and TNF-α levels were down-regulated significantly in dose groups, with statistical significance （P〈0.05）. Meanwhile, Kangshuai Yizhi Capsule high-dose group showed the most obvious effect among dose groups.ConclusionKangshuai Yizhi Capsule has effects of enhancing activity of ATP in brain tissue and reducing level of proinflammatory factors.