文献类型：期刊文献

英文题名：Angelica sinensis polysaccharide promotes apoptosis by inhibiting JAK/STAT pathway in breast cancer cells

作者：Fu, Zhaoyuan[1];Li, Yingdong[1];Yang, Suisheng[2];Ma, Chengxu[1];Zhao, Rongrong[3];Guo, Huan[1];Wei, Huiping[1]

第一作者：付兆媛

通信作者：Li, YD[1]

机构：[1]Gansu Univ Tradit Chinese Med, Dept Integrated Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Canc Hosp, Dept Breast Oncol, Lanzhou 73005, Gansu, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Affiliated Hosp, Dept Clin Lab Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Dept Integrated Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2019

卷号：18

期号：11

起止页码：2247

外文期刊名：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

收录：;Scopus(收录号：2-s2.0-85078033357);WOS:【SCI-EXPANDED(收录号:WOS:000500260000003)】；

基金：This work was supported by National Natural Science Foundation of China (Grant no. 8187140840), and Science and Technology Achievement Transformation Project of Education Department of Gansu Province (Grant no. 2016D-11).

语种：英文

外文关键词：Angelica polysaccharide; Apoptosis; Breast cancer; JAK; STAT

摘要：Purpose: To determine whether Angelica polysaccharide (APS) induced apoptosis via regulation of the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) pathway in breast cancer cells. Methods: Human MCF-7 cells were treated with APS. Cell proliferation, apoptosis, expression of apoptotic proteins, and the phosphorylation level of Janus kinase (JAK) and STAT were measured, respectively. For further analysis, MCF-7 cells were transfected with a JAK2 overexpression plasmid or treated with a classical JAK inhibitor, ruxolitinib. Results: Treatment with APS dose-dependently reduced cell proliferation, induced apoptosis, and downregulated the levels of phosphorylated JAK and STAT in MCF-7 cells. JAK inhibitor, ruxolitinib, blocked JAK/STAT pathway and induced cell apoptosis in MCF-7 cells. Besides, JAK2 overexpression reversed the effects of APS on cell viability and apoptosis. Conclusion: The results indicate that polysaccharide isolated from Angelica sinensis promotes apoptosis by inhibiting JAK/STAT pathway in breast cancer cells. Thus, APS may be useful as a potential therapeutic agent for breast cancer.