文献类型：期刊文献

中文题名：镰形棘豆生物碱对临床9种致病菌的抑制作用研究

英文题名：Antibacterial Activity of Alkaloids Extract from Oxytropis falcata Bunge Against 9 Pathogens

作者：姜华[1];胡君茹[1];张丽[2]

第一作者：姜华

机构：[1]甘肃省中医药研究院,甘肃兰州730050;[2]甘肃中医学院,甘肃兰州730000

第一机构：甘肃省中医药研究院,甘肃兰州730050

年份：2014

卷号：21

期号：2

起止页码：53

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金(81260633)

语种：中文

中文关键词：镰形棘豆;生物碱;致病菌;抑菌作用

外文关键词：Oxytropis falcata Bunge; alkaloids extract; pathogens; antibacterial activity

摘要：目的观察镰形棘豆生物碱提取物对9种常见致病菌的抗菌作用。方法以镰形棘豆为原料，提取生物碱，选择临床常见的4种革兰氏阳性菌和5种革兰氏阴性菌，采用琼脂扩散法对其体外抑菌活性进行评价，试管二倍稀释法测定其最低抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果镰形棘豆生物碱提取物对9个菌种均有抑菌效果，抑菌作用敏感度与浓度呈良好的量效关系，其中对枯草杆菌和嗜麦芽窄食单胞菌的抑菌效果优于其他各菌，MIC和MBC分别为0．95mg／mL和1．91mg／mL，而其他各菌种的MIC和船C范围在1．91～7．63mg／mL之间。结论镰形棘豆生物碱提取物具有较为广谱的抑菌作用，对革兰氏阳性菌的抑制作用比革兰氏阴性菌强。
Objective To investigate the antibacterial activity of the alkaloids extract from Oxytropis falcata Bunge against 9 common pathogens in vitro. Methods Alkaloids was extracted from Oxytropis faleata Bunge. The agar plate dilution method was adopted to evaluate the activity against bacteria of 4 Gram-negative test strains and 5 Gram-positive test strains, while the MIC and MBC were determined by tube double dilution method. Results The alkaloids extract from Oxytropis falcata Bunge had the antibacterial activity against 9 pathogens and there was a good dose-response relationship between sensitivity and concentration. Antibacterial activities against bacillus subtilis and stentrophmomas maltophilia showed stronger than other tested bacteria, the MIC and MBC were 0.95 mg/mL and 1.91 mg/mL, respectively. In other tested bacteria, MIC and MBC were ranged in 1.91-7.63 mg/mL. Conclusion The alkaloids extract showed a broad spectrum of a stronger antibacterial activity against all tested pathogens. Gram-positive strains were more sensitive than Gram-negative strains to the inhibitory effects of the alkaloids extract.