文献类型：期刊文献

英文题名：Flos puerariae ameliorates the intestinal inflammation of Drosophila via modulating the Nrf2/Keap1, JAK-STAT and Wnt signaling

作者：Yang, Shipei[1,2,3];Li, Xu[4];Xiu, Minghui[1,2,4,5];Dai, Yuting[4];Wan, Shengfang[3];Shi, Yan[1,2,3];Liu, Yongqi[1,2,6];He, Jianzheng[1,2,3,6]

第一作者：Yang, Shipei

通信作者：Liu, YQ[1];He, JZ[1];Liu, YQ[2];He, JZ[2];He, JZ[3];Liu, YQ[4];He, JZ[4]

机构：[1]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Gansu Univ Chinese Med & Univ, Prevent & Treatment Tradit Chinese Med Res Gansu C, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Coll Basic Med, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[5]Gansu Univ Chinese Med, Res Ctr Tradit Chinese Med Gansu, Lanzhou, Peoples R China;[6]Gansu Univ Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial L, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Gansu Univ Chinese Med & Univ, Prevent & Treatment Tradit Chinese Med Res Gansu C, Lanzhou, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Coll Basic Med, Lanzhou, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial L, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;[107351d2d02a88e1f325f]甘肃中医药大学基础医学院（敦煌医学研究所）;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85137810691);WOS:【SCI-EXPANDED(收录号:WOS:000848532200001)】；

基金：The authors appreciated the financial support received from the National Natural Science Foundation of China (Nos. 82104562, 82004228), Provincial University industry support project in Gansu (2020C-15), Foundation from Research Center of Traditional Chinese Medicine in Gansu (No. zyzx-2020-05).

语种：英文

外文关键词：Flos Puerariae; intestinal inflammation; intestinal homeostasis; Drosophila melanogaster; intestinal stem cells; ROS

摘要：Gut homeostasis is important for human health, and its disruption can lead to inflammatory bowel disease (IBD). Flos Puerariae is a herb with a wide variety of pharmacological activities including antioxidant, antidiabetic, antialcoholismic and anti-inflammatory properties. However, the role of Flos Puerariae on treating IBD remains obscure. Here, we employed Drosophila melanogaster as a model organism to investigate the protective effect of Flos Puerariae extract (FPE) against sodium dodecyl sulfate (SDS)-induced intestinal injury. Our data showed that FPE had no toxic effect in flies, and significantly extended lifespan in SDS-inflamed flies, reduced stem cell proliferation in the midgut, and maintained intestinal morphological integrity. Furthermore, FPE remarkably recused the altered expression level of genes and proteins in Nrf2/Keap1 signaling, JAK-STAT signaling and Wnt signaling pathways in gut of inflammation flies. Thus, FPE has a protective effect against intestinal injury possibly via increasing the Nrf2/keap1 pathway and suppressing the JAK-STAT and Wnt signaling pathways, which would have tremendous potential for treating IBD.