文献类型：期刊文献

英文题名：The Mechanism of Qishen Yixin Granules in Suppressing Myocardial Fibrosis: A Pharmacological Validation Based on Network Pharmacology

作者：Jiang, Hugang[1];Wang, Xinqiang[1];Lin, Wenyan[2];Liu, Kai[1];Li, Yingdong[1];Zhao, Xinke[3]

第一作者：Jiang, Hugang

通信作者：Li, YD[1];Zhao, XK[2]

机构：[1]Gansu Univ Chinese Med, Dept Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Peoples Hosp, Daytime Diag & Treatment Ctr, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Cardiovasc Clin Med Ctr, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Dept Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Cardiovasc Clin Med Ctr, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China.|[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;[10735]甘肃中医药大学;

年份：2023

卷号：37

期号：7

起止页码：3541

外文期刊名：JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS

收录：;Scopus(收录号：2-s2.0-85204744588);WOS:【SCI-EXPANDED(收录号:WOS:001050594100001)】；

基金：This work was supported by the National Natu-ral Science Foundation of China (81860786), the Gansu Provincial Department of Education Unveiling and Leading Project (2021jyjbgs-03), the Capacity Building Project of Chinese Medicine (2019XZZX-XXG002) , Gansu University of Chinese Medicine Science and Technology Innovation Project (2021KCYB-5) .

语种：英文

外文关键词：Qishen Yixin Granules (QSYXG); myocardial fibrosis (MF); network pharmacology

摘要：Background: Myocardial fibrosis (MF) is a condition that plays a crucial role in heart failure (HF) development and death, and current drugs for its treatment are limited. Qishen Yixin Granule (QSYXG) is a Traditional Chinese Medicine (TCM) that has been shown to be effective in treating chronic HF, but its mechanism of action is not yet fully understood. This study aimed to reveal the molecular mechanisms and bioactive compounds of QSYXG treating MF using an integrated network pharmacology and pharmacological validation pathway.Methods: The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database, drug-target database and GeneCard database were used to screen active ingredients, drug targets and MF genes. The clusterProfiler package in R programming language was employed for functional and pathway enrichment analyses. Experimental valida-tion was completed using hematoxylin-eosin staining, Masson's trichrome staining, and immunohistochemistry in isoproterenol-induced MF rats. Western blot, phalloidin staining, and immunofluorescence staining were performed to elucidate the predicted mechanism on H9C2 cells.Results: In this study, 55 bioactive components and 59 putative targets were collected. Functional enrichment analysis revealed that responses to lipopolysaccharides, oxidative stress, and hypoxia constituted vital biological processes. Six targets, contain-ing mitogen-activated protein kinase (MAPK) 14, prostaglandin-endoperoxide synthase 2 (PTGS2), serine/threonine kinase 1 (AKT1), MAPK8, Interleukin (IL)-6 and IL-1 & beta;, directly regulated these responses simultaneously. Five pathways were identi-fied by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. QSYXG could downregulate the expression of PTGS2, MAPK14 and MAPK8 and upregulate the expression of AKT1 in the treatment of MF.Conclusions: This study revealed that QSYXG could alleviate MF based on multiple components, targets and pathways.