文献类型：期刊文献

中文题名：rRNA基因间隔区碱基测序对当归进行鉴定的研究

英文题名：Identification by measuring internal transcribed spacer regions of rRNA gene in Radix Angelicae Sinensis

作者：姬可平[1];李应东[2];张西玲[3];李啸红[1]

第一作者：姬可平

机构：[1]甘肃中医学院生物学教研室,甘肃兰州730000;[2]甘肃中医学院中心实验室,甘肃兰州730000;[3]甘肃中医学院中药鉴定教研室,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2003

卷号：34

期号：1

起止页码：66

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：甘肃省自然科学基金资助项目 (ZS0 11-A2 5 -0 5 8-Y)

语种：中文

中文关键词：当归;套式PCR;DNA测序;rRNA基因;分子鉴定

外文关键词：Radix Angelicae Sinensis; nested PCR; to measure the sequence of DNA; rRNA gene; molecular identification

摘要：目的　通过 r RNA基因内转录间隔区的碱基序列测定 ,对当归进行分子水平鉴定。方法　常规提取当归种籽 DNA,利用合成的特异性引物对其 r RNA基因内转录间隔区进行套式 PCR扩增 ,将扩增产物进行碱基序列测定。结果　琼脂糖凝胶电泳证实当归种籽中 r RNA基因内转录间隔区 PCR扩增产物存在 ;经测序后得到了当归种籽 r RNA基因内转录间隔区的碱基序列。结论　 r RNA基因内转录间隔区的碱基序列测定可做为分子水平鉴定植物性中药材的又一有效途径。
Object To set up an identified standard on the level of molecule through measuring the internal transcribed spacer regions of the rRNA gene in Radix Angelicae Sinensis Methods To extract DNA from the seeds of Radix Angelicae Sinensis by conventional method, and use composed peculiar primer amplify with the internal transcribed spacer regions of the rRNA gene, to measure the base sequence of the amplified products Results It is proved by agar sugar gel electrophoresis that the PCR amplified products of the internal transcribed spacer regions of the rRNA gene exist The base sequence of the seeds of Radix Angelicae Sinensis internal transcribed spacer regions of the rRNA gene was measured Conclusion To measure the base sequence of internal transcribed spacer regions of the rRNA gene is another effective method to identify the vegetal traditional Chinese medicine on the molecular level