文献类型：期刊文献

中文题名：肝郁脾虚证大鼠血细胞和脏器指数改变的实验研究

英文题名：Experimental research of blood cell and organ index on syndrome of liver depression and spleen deficiency of rats

作者：刘建鸿[1];颜春鲁[1];岳嘉[1];赵宁[1];陈继锋[1]

第一作者：刘建鸿

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2012

卷号：29

期号：5

起止页码：8

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

基金：甘肃省科技厅自然科学基金资助项目(0803RJZA071)

语种：中文

中文关键词：肝郁脾虚证;大鼠;外周血细胞;脏器指数

外文关键词：syndrome of liver depression and spleen deficiency; rats; peripheral blood cell; organ index

摘要：目的探讨实验条件下肝郁脾虚证大鼠各类血细胞和脏器指数的改变情况。方法采用夹尾激怒、束缚应激、过度疲劳与饮食失节的复合方法复制肝郁脾虚证动物模型,检测对照组和2周、6周、9周模型组雄性大鼠外周血象和胸腺、肾上腺、肝、脾等脏器指数。结果外周血象检测结果显示,与空白对照组比较,肝郁脾虚证大鼠2周组白细胞和中性粒细胞数量升高(P<0.05或P<0.01),9周组红细胞数量、血红蛋白含量和淋巴细胞数量降低(P<0.05);脏器指数检测结果显示,与空白对照组比较,肝郁脾虚证大鼠6周、9周组胸腺指数降低(P<0.01),9周组肾上腺、脾脏和肝脏指数升高(P<0.05)。结论肝郁脾虚因素会影响机体的血液系统,机体遭受应激刺激时虽然短期能使中性粒细胞的数量增加以对抗伤害,增强抵抗力,但长期肝失疏泄、脾失健运导致神经-内分泌-免疫网络紊乱,造成贫血和淋巴细胞数量减少,机体运氧能力和抵抗力降低。
Objective To explore the changes of syndrome of liver depression and spleen deficiency for all kinds of blood cell and organ index.Methods In addition to the normal group,other groups were devised model of syndrome of liver depression and spleen deficiency rats which were angered by clipping tails,restraint stress,over fatigue and abnormal diet.Measured the peripheral blood cell,thymus index,adrenal gland,hepatic index and spleen index in the normal group and other model groups on 2 weeks,6 weeks,9 weeks.Results The results of peripheral blood showed： while the quantity of leukocyte and neutrophils in syndrome of liver depression and spleen deficiency of rats on 2 weeks was significantly higher than those in normal group（P0.05 or P0.01）,the quantity of red blood cell,hemoglobin and lymphocyte in model group on 9 weeks was significantly lower than those in normal group（P0.05）.The results of organ index showed： while the thymus index in syndrome of liver depression and spleen deficiency of rats on 6 weeks and 9 weeks was significantly lower than those in normal group（P0.01）,the adrenal gland,hepatic index and spleen index in model group on 9 weeks was significantly higher than those in normal group（P0.05）.Conclusion The factor of liver depression and spleen deficiency can affect the blood system,the body suffer from stress stimulation,short-term can increase in the number of neutrophils in the battle against damage factors effect,increase the resistance;But long-term of liver,spleen pathogenesis of health luck cause nerve-endocrine network disorders,that cause anemia,the quantity of lymphocyte decreased and the ability of body transport oxygen and immunity are decreased.