文献类型：期刊文献

英文题名：SIMULTANEOUS DETERMINATION OF FOUR FLAVONOID AGLYCONE ANALOGS IN THE INFLORESCENCE OF JUGLANS REGIA L. BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTION

作者：Zhao, Lei[1];Li, Jintian[2];Wang, Tongwen[3];Liu, Lina[1]

第一作者：赵磊

通信作者：Zhao, L[1]

机构：[1]Gansu Coll Tradit Chinese Med, Coll Gansu Prov, Lab Chem & Qual Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Minist Educ, Key Lab Dunhuang Med & Transformat, Lanzhou 730000, Peoples R China;[3]Univ North Carolina Chapel Hill, Inst Nutr Res, Kannapolis, NC USA

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Coll Gansu Prov, Lab Chem & Qual Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2013

卷号：36

期号：14

起止页码：1986

外文期刊名：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000319553100007)】；

基金：The authors gratefully acknowledge the Fund of Scientific and Technical research of Traditional Chinese Medicine of Gansu province (GZK-2010-57) and the Fund of the Scientific and Technical Innovation Project of Gansu province (1104FKCA122) for financially supporting this research. The authors thank Prof. Chong Li for providing reference standards.

语种：英文

外文关键词：flavonoid aglycones analogs; high-performance liquid chromatography-diode array detection; high-performance liquid chromatography- electrospray tandem mass spectrometry; Juglans regia L; simultaneous determination

摘要：A reversed-phase high-performance liquid chromatography coupled with a diode array detection method and a high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method were first developed and validated for the simultaneous determination of four flavonoid aglycone analogs in the inflorescence of Juglans regia L. grown in different areas of Gansu province, China, surmounting the challenges rising from the complexity of the matrices owing to different origins of the plant. The separation of the four compounds was accomplished through a ternary mobile phase, with good specificity and satisfactory resolution. The chromatographic peaks of the four investigated compounds were identified by comparing their retention time and mass spectrometric profile with the corresponding reference compounds. The method showed a good linearity (r(2)>0.9998), high precision (RSD <5%), and recoveries (ranged from 96.6-102.7%) were acceptable. The limits of detection (LOD) and the limits of quantification (LOQ) are ranged from 0.26-0.58 mu g/ml and from 0.86-1.90 mu g/ml, respectively. The results demonstrated that this analytical approach is ideal for the determination of such bioactive compounds as flavonoid aglycones, and that it constructed a basis for the comprehensive evaluation of the quality of Juglans regia L.