文献类型：期刊文献

中文题名：贞芪酪蛋白肽复合物血清对人肺腺癌A549细胞分化抑制作用及相关机制研究

英文题名：Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study

作者：张延英[1];吴建军[1];王鹏善[2];薛娜[1];舒畅[1];吴红彦[1]

第一作者：张延英

机构：[1]甘肃中医学院,兰州730000;[2]高台县宣化乡卫生院,甘肃张掖734304

第一机构：甘肃中医药大学

年份：2010

卷号：16

期号：4

起止页码：114

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2008】；

语种：中文

中文关键词：贞芪酪蛋白肽复合物;药物血清;A549;细胞分化;凋亡

外文关键词：Ligustri-Astragali Casein co-peptid（LACP）; drug serum; human Lung adenocarcinoma A549 cell; cell differentiation; apoptosis

摘要：目的:探索贞芪酪蛋白肽(ligustri-astragali casein co-peptid,LACP)复合物对人肺腺癌A549细胞分化的诱导作用及作用机制。方法:MTT法检测贞芪酪蛋白肽含药血清5%,10%,15%,20%浓度对人肺腺癌A549细胞增殖的影响;通过贞芪酪蛋白肽药物血清对A549细胞核质比和端粒酶活性的影响观察其诱导分化作用;免疫细胞化学方法观察贞芪酪蛋白肽药物血清对A549细胞Bax/bcl-2基因表达的影响。结果:4种不同浓度的贞芪酪蛋白肽含药血清均对A549细胞增殖有抑制作用,其中以浓度为20%的贞芪酪蛋白肽血清的抑制率最高;能降低细胞的核质比,并抑制人肺癌细胞端粒酶活性的作用;贞芪酪蛋白肽血清能促进A549细胞凋亡基因bax/bcl-2的表达,与无药血清对照组比较,差异有统计学意义(P<0.01)。结论:贞芪酪蛋白肽含药血清对人肺腺癌细胞A549有一定抑制作用;有诱导人肺癌细胞A549分化的作用,其主要机理可能是抑制了A549细胞端粒酶活性;贞芪酪蛋白肽诱导A549细胞凋亡的作用机制之一可能是促进促凋亡基因bax/bcl-2的表达。
Objective：To explore the effect of ligustri-astragali casein co-peptid（LACP） on human lung adenocarcinoma A549 cell differentiation and the related mechanism.Method： MTT assay for human lung adenocarcinoma A549 cells;containing 5%,10%,15%,20% concentration of LACP serum was used.The influence of LACP containing serum on differentiation was evaluated by investigation of cellular nuclear-mass ratio and telomerase activity.Immunocytochemical method was used to observe the effect of LACP drug serum on the A549 Bax /bcl-2 expression.Results： The four kinds of different concentrations of LACP serum inhibited A549 cell proliferation.Among them,20% of LACP showed the highest inhibitory rate;and reduced the cell＇s nuclear-mass ratio.LACP serum promoted A549 cell bax / bcl-2 gene expression for apoptosis,and compared with the serum-free control group,the difference was significant（P 0.05）.Conclusion： LACP containing serum has certain inhibitory effect on human lung adenocarcinoma A549 cells.LACP complex shows promotion to human lung cancer cell A549 differentiation.The mechanism may be linked to inhibition of telomerase activity.The complex induced apoptosis of A549 cells may be related to promotion of bax /bcl-2 gene expression.