文献类型：期刊文献

中文题名：黄芪多糖对X线辐射骨髓间充质干细胞向成骨方向分化的影响

英文题名：Effects of Astragalus Polysaccharide on Osteoblastic Differentiation of Bone Mesenchymal Stem Cells Induced by X-ray Radiation

作者：王磊[1];卢志伟[1];许小敏[1];刘永琦[1,2];张利英[1];张苡铭[1];张丽昕[1];何进鹏[3];丁楠[3]

第一作者：王磊

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000;[3]甘肃省空间辐射生物学重点实验室,中国科学院近代物理研究所,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：24

期号：12

起止页码：47

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81473457)

语种：中文

中文关键词：黄芪多糖;X线辐射;骨髓间充质干细胞;成骨分化

外文关键词：astragalus polysaccharide; X-ray radiation; BMSCs; osteoblasts differentiation

摘要：目的观察黄芪多糖(APS)对X线辐射骨髓间充质干细胞(BMSCs)向成骨方向分化的影响,探讨其相关作用机制。方法采用CCK-8法检测不同浓度APS对2 Gy X线辐射BMSCs细胞增殖能力的影响,以筛选最佳药物浓度。将细胞分为空白组、APS组、辐射组、辐射+APS组。APS组、辐射+APS组用最佳药物浓度APS提前干预3 d,辐射组、辐射+APS组用2 Gy X线进行辐射,辐射后各组细胞均加2 m L成骨诱导液继续培养,每3 d换液1次。诱导15 d后,镜下观察细胞形态,茜素红染色检测细胞中钙结节面积,Western blot检测细胞中特异性标记蛋白骨桥蛋白(OPN)和骨钙蛋白(OCN)的表达。结果与空白组比较,辐射组细胞增殖水平明显降低(P<0.05);与辐射组比较,辐射+APS组细胞增殖水平明显升高(P<0.05);APS浓度为50μg/m L时其促进增殖作用最强,为最佳药物浓度。形态学观察显示,辐射组细胞中钙结晶沉积较空白组和APS组明显减少,辐射+APS组细胞中钙结晶沉积较辐射组明显增加。在成骨能力方面,与空白组比较,APS组细胞中钙结节面积有一定降低,而辐射组细胞中钙结节面积显著降低(P<0.05);与辐射组比较,辐射+APS组细胞中钙结节面积明显增多(P<0.05)。在成骨特异性标记蛋白表达方面,与空白组比较,APS组细胞中OPN表达略下降,OCN表达略升高;辐射组细胞中OPN和OCN表达均明显下调(P<0.05);与辐射组比较,辐射+APS组细胞中OPN和OCN表达均显著升高(P<0.05)。结论黄芪多糖对X线辐射BMSCs向成骨方向分化的潜能具有保护作用。
Objective To investigate the effects of astragalus polysaccharide(APS)on bone mesenchymal stem cells(BMSCs)to osteoblasts differentiation induced by X-rays;To discuss its mechanism of action.Methods CCK-8method was used to select different concentrations of APS for the proliferation ability of BMSCs with2Gy X-ray radiation,and the best concentration was determined.Cells were divided into blank group,APS group,radiation group,radiation+APS group.APS group and radiation+APS group were given the best concentration of APS for3days,radiation group and radiation+APS group were given2Gy X-ray radiation.After radiation,2mL osteogenesis induced liquid was added in each group,every3day.After15day's induction,inverting microscope was used to observe morphology,and alizarin red staining to detect the area of the calcium nodules in each group.Western blot was used to detect the specific marker protein osteopontin and osteocalcin expression of each group.Results Compared with the blank group,the proliferation ability of radiation group was obviously lower(P<0.05);compared with radiation group,the proliferation ability of radiation+APS significantly increased(P<0.05);the strongest promoting proliferation of APS was50μg/mL,therefore,it was selected as the best concentration.In terms of morphology,inverted microscope showed that secretion of crystals of radiation group was obviously reduced compared with the blank group and APS group,and secretion of crystals of radiation+APS group was significantly elevated compared with radiation group.In osteogenesis ability,compared with the blank group,the cell calcium nodule area of APS group had a certain reduce,but the radiation group had a significantly reduced(P<0.05).Compared with the radiation group,the cell calcium nodule area of radiation+APS group obviously increased(P<0.05).In terms of osteogenesis specific marker protein expression,compared with the blank group,the expression of osteopontin of APS group was slightly declined,and the expression of osteocalcin was slightly elevated,but the expression of osteopontin and osteocalcin of radiation group was significantly lowered(P<0.05).Compared with the radiation group,the expression of osteopontin and osteocalcin of radiation+APS group was significantly higher(P<0.05).Conclusion APS has protective effects on osteoblastic differentiation ablility of BMSCs induced by X-ray radiation.