文献类型：期刊文献

英文题名：The total alkaloids of Aconitum tanguticum protect against lipopolysaccharide-induced acute lung injury in rats

作者：Wu, Guotai[1];Du, Lidong[2];Zhao, Lei[2];Shang, Ruofeng[1];Liu, Dongling[2];Jing, Qi[2];Liang, Jianping[1];Ren, Yuan[2]

第一作者：Wu, Guotai

通信作者：Liang, JP[1]

机构：[1]Chinese Acad Agr Sci, Lanzhou Inst Anim Sci & Vet Pharmaceut Sci, Key Lab Vet Pharmaceut Discovery, Key Lab New Anim Drug Project Gansu Prov,Minist E, Lanzhou 730050, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou 730000, Gansu, Peoples R China

第一机构：Chinese Acad Agr Sci, Lanzhou Inst Anim Sci & Vet Pharmaceut Sci, Key Lab Vet Pharmaceut Discovery, Key Lab New Anim Drug Project Gansu Prov,Minist E, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), CAAS, Lanzhou Inst Husb & Pharmaceut Sci, 335 Jiangouyan Rd, Lanzhou 730050, Peoples R China.

年份：2014

卷号：155

期号：3

起止页码：1483

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000343380300009)】；

基金：This study was supported by the "Five-Year" plan for national science and technology projects in rural areas of China (No. 2011AA10A214) and the Program of Gansu Province Key Laboratory of Pharmacology and Toxicology of TCM (No. ZDSYS-KJ-2012-004). The authors are grateful to Dr. Yali She for providing technical assistance in pathological observations and to Dr. Xiaoming Sun for English language revisions.

语种：英文

外文关键词：Total alkaloids of Aconitum tanguticum; Acute lung injury; TNF-alpha; IL-6; IL-1 beta; NF-kappa B

摘要：Ethnopharmacological relevance: Aconitum tanguticum has been widely used as a remedy for infectious diseases in traditional Tibetan medicine in China. The total alkaloids of Aconitum tanguticum (TAA) are the main active components of Aconitum tanguticum and have been demonstrated to be effective in suppressing inflammation. Our aim was to investigate the protective effects of TAA on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Materials and methods: TAA was extracted in 95% ethanol and purified in chloroform. After vacuum drying, the TAA powder was dissolved in dimethyl sulfoxide. Adult male Sprague-Dawley rats were randomly divided into six groups. Rats were given dexamethasone (DXM, 4 mg/kg) or TAA (60 mg/kg, 30 mg/kg) before LPS injection. The PaO(2)and PaO2/FiO(2) values, lung wet/dry (W/D) weight ratio and histological changes in lung tissue were measured. The cell counts, protein concentration, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) in bronchoalveolar lavage fluid (BALF), and myeloperoxidase (MPO) activity in lung tissue were determined at 6, 12 or 24 h after LPS treatment. In addition, the NF-kappa B activation in lung tissue was analyzed by western blot. Results: In ALI rats, TAA significantly reduced the lung W/D ratio and increased the value of PaO2 or PaO2/FiO(2) at 6, 12 or 24 h after LPS challenge. TAA also reduced the total protein concentration and the number of total cells, neutrophils or lymphocytes in BALF. In addition, TAA decreased MPO activity in the lung and attenuated histological changes in the lung. Furthermore, TM inhibited the concentration of TNF-alpha, IL-6 and IL-1 beta in BALF at 6, 12 or 24 h after LPS treatment. Further study demonstrated that TM significantly inhibited NF-kappa B activation in lung tissue. Conclusions: The current study proved that TAA exhibited a potent protective effect on LPS-induced ALI in rats through its anti-inflammatory activity. (C) 2014 Elsevier Ireland Ltd. All rights reserved.