文献类型：期刊文献

英文题名：Isolation and characterization of 18 polymorphic microsatellite markers for the "Female Ginseng" Angelica sinensis (Apiaceae) and cross-species amplification

作者：Lu, Youyuan[1,2];Cheng, Tao[3];Zhu, Tiantian[1];Jiang, Dan[2];Zhou, Shiliang[3];Jin, Ling[1];Yuan, Qingjun[2];Huang, Luqi[2]

第一作者：Lu, Youyuan

通信作者：Huang, LQ[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China;[3]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China.

年份：2015

卷号：61

起止页码：488

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000361164800068)】；

基金：The authors especially thank Jun-Jie Guo for help with data analyses and manuscript preparation. This research was supported by the National Natural Science Foundation of China (NSFC: 81360615, 81274027 and 81130070), Beijing Joint Project Specific Funds and the Independent Project of Basic Scientific Research Operating Expenses of Institute of Chinese Materia Medial, China Academy of Chinese Medical Sciences (2011ZDXK-01).

语种：英文

外文关键词：Angelica sinensis; Medicinal plant; Microsatellite; Molecular marker; Cross-amplification; Population genetics

摘要：Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved.