文献类型：期刊文献

英文题名：Intermittent blue light-dark cycles: A new strategy for enhancing triterpenoid production in medicinal Sanghuangporus vaninii

作者：Ma, Yanjun[1];Li, Rong[1];Li, Jiahui[1];Song, Zilu[1];She, Fawen[2];Ma, Xiaoyan[3];Zhu, Xuetai[1]

第一作者：Ma, Yanjun

通信作者：Ma, YJ[1]

机构：[1]Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China;[2]Baiyin 1 High Sch, Baiyin, Peoples R China;[3]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou, Peoples R China

第一机构：Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China.

年份：2026

卷号：274

外文期刊名：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY

收录：;Scopus(收录号：2-s2.0-105024421390);WOS:【SCI-EXPANDED(收录号:WOS:001642074600001)】；

基金：This work was kindly supported by the National Natural Science Foundation of China (32500047) and the Young Doctor Fund Project of Education Department of Gansu Province (2024QB-016) .

语种：英文

外文关键词：Medicinal fungus; Photoperiod; 3-hydroxy-3-methylglutaryl-CoA synthase; Membrane permeability; Race technology

摘要：Triterpenoids from medicinal fungi have significant biological activities and industrial potential, but their low yield limits large-scale production. Sanghuangporus vaninii, a widely cultivated medicinal fungus, is a promising source of triterpenoids, yet the regulatory role of light-dark cycles in its triterpenoid biosynthesis remains unclear. In this study, we demonstrated that intermittent intense blue light-dark cycles (IB/D; similar to 110 mu mol/m(2)center dot s, 10 h/14 h) dramatically enhance triterpenoid production in S. vaninii MF5. IB/D treatment increased triterpenoid yield by 154 % compared to dark controls, also outperforming constant light or white light-dark regimens. Mechanistically, IB/D was associated with: (i) hyperbranching morphology (42 % reduced internode length); (ii) membrane remodeling via upregulated fatty acid desaturases and transporters, enhancing permeability 2.88-fold; and (iii) transcriptional activation of the mevalonate pathway, which was accompanied by a dramatic induction of endogenous 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) expression 4497-fold. Subsequent cloning and characterization of the SvHMGS gene-through sequence homology with known HMGS genes from Sanghuangporus species, identification of conserved HMGS domains, and prediction of its association with the mevalonic acid (MVA) pathway-suggested its potential conserved function in triterpenoid biosynthesis. This photoperiodic strategy represents a paradigm shift from constant light suppression to rhythmic induction, offering a scalable, low-cost approach to boost triterpenoid production in this medicinal fungus.