文献类型：期刊文献

英文题名：Establishment and evaluation of a stable and reliable rat model of peritoneal adhesions

作者：Nian, Hongyu[1,2];Pu, Zhenjun[1,2];Li, Zhiyuan[1];Zhong, Pengfei[1];Ma, Shengxu[1];Li, Junliang[1,2,3,4]

第一作者：Nian, Hongyu

通信作者：Li, JL[1]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China;[2]Gansu Prov Hosp, Dept Gen Surg, 204 Donggang West Rd, Lanzhou 730030, Gansu, Peoples R China;[3]Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gansu, Lanzhou, Peoples R China;[4]Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Gen Surg, 204 Donggang West Rd, Lanzhou 730030, Gansu, Peoples R China.

年份：2024

卷号：176

期号：4

起止页码：1256

外文期刊名：SURGERY

收录：;Scopus(收录号：2-s2.0-85199100003);WOS:【SCI-EXPANDED(收录号:WOS:001310771900001)】；

基金：This work was supported by the First Batch of Longyuan Youth Talent Project in Gansu Province (001068001) , the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (NHCDP2022028) , Key Laboratory of Molecular Di-agnostics and Precision Medicine for Surgical Oncology in Gansu Province (2020GSZDSYS02) , Research Fund project of Gansu Pro-vincial Hospital (22GSSYC-1, 22GSSYB-14) , and Key Laboratory of Dunhuang Medicine, Ministry of Education (DHYX22-02) .

语种：英文

摘要：Background: In this study, we aimed to establish a stable and standardized animal model of peritoneal adhesions. Methods: Forty-eight male Sprague-Dawley rats were randomly divided (n = 12 each) into blank control, classic cecum sidewall, ischemic button, and cecum-sidewall suture groups. The modified American Fertility Society adhesion score was used on postoperative day 7 to evaluate adhesions. Sixty male Sprague-Dawley rats were used to dynamically observe the adhesion characteristics of cecum-sidewall ischemic injury suture model at different time points (n = 60, randomly divided into groups a-e with 12 rats each). The modified American Fertility Society and Z & uuml;hlke histologic scoring systems, hematoxylineosin staining, Masson staining, and computed tomography of the abdomen were used to evaluate adhesions on postoperative days 1, 3, 5, 7, and 14. Results: No peritoneal adhesions were observed in the blank control group on postoperative day 7. In the classic cecum sidewall group, 8 rats had inconsistent adhesions, which had a modified American Fertility Society adhesion score of 2.25 +/- 1.96. All rats in the ischemic button and cecum-sidewall suture groups developed significant adhesions with modified American Fertility Society scores of 3.08 +/- 1.31 and 4.67 +/- 0.78, respectively. When the modified American Fertility Society score was used, statistically significant differences were observed between the classic cecum sidewall groups and cecum-sidewall suture groups and between the ischemic button groups and cecum-sidewall suture groups. All animals in groups a-e developed adhesions; adhesion scores increased gradually with time. Conclusions: The cecum-sidewall ischemic injury suture model is a stable and standardized animal model of peritoneal adhesions.
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