文献类型：期刊文献

英文题名：Identification of byakangelicin metabolites in rats via the UHPLC-Q-exactive orbitrap mass spectrometer

作者：Cai, Ruijun[1];Mao, Zhongnan[2];An, Tao[1];Zhang, Yunbo[3];Zhou, Li[1]

第一作者：Cai, Ruijun

通信作者：Zhou, L[1]

机构：[1]Shanghai Gen Hosp, Jiuquan Hosp, Peoples Hosp Jiuquan, Dept Pharm, Jiuquan 735000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Shanghai Gen Hosp, Jiuquan Hosp, Peoples Hosp Jiuquan, Dept Pharm, Jiuquan 735000, Gansu, Peoples R China

通信机构：[1]corresponding author), Shanghai Gen Hosp, Jiuquan Hosp, Peoples Hosp Jiuquan, Dept Pharm, Jiuquan 735000, Gansu, Peoples R China.

年份：2026

卷号：1268

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001618017900001)】；

基金：Acknowledgments This work was supported by the Scientific research project of Gansu Provincial Administration of Traditional Chinese Medicine (project number: GZKG-2024-84) .

语种：英文

外文关键词：Byakangelicin; Metabolites; In vivo; UHPLC-Q-exactive orbitrap MS; Sprague-Dawley rats

摘要：Byakangelicin is a furanocoumarin derived from the roots of Angelica dahurica. It exhibits pharmacological properties, including anti-tumor activity, as well as against liver injury and fibrosis. In this study, Ultra-High-Performance Liquid Chromatography with Q-Exactive Orbitrap Mass Spectrometry technology was employed to investigate the metabolic profile of byakangelicin in rats. Following intragastric administration the suspension of byakangelicin, plasma and tissue specimens were harvested. According to high-resolution extracted ion chromatograms, the metabolites of byakangelicin were identified by comparing accurate mass, diagnostic fragment ions, and chromatographic retention times. Data collection was performed in positive ion mode to facilitate metabolite characterization of byakangelicin. Overall, 46 metabolites were successfully characterised. The primary metabolic pathways included hydroxylation, dehydroxylation, methylation, demethylation, reduction, glucuronidation, glycination, and cysteinylation. This systematic investigation of the metabolic characteristics and pathways of byakangelicin in rats provides a valuable reference for future pharmacodynamic evaluations, pharmacological research, and drug development.