文献类型：期刊文献

中文题名：当归补气活性部位的谱效关系研究

英文题名：Spectrum-effect relationship of active fraction from Angelicae Sinensis Radix with effect of reinforcing Qi

作者：杨英来[1];胡芳[1];刘小花[1];郭龙[1];杨涛[1];李应东[2];封士兰[1]

第一作者：杨英来

机构：[1]兰州大学药学院,甘肃兰州730000;[2]甘肃中医学院,甘肃兰州730000

第一机构：兰州大学药学院,甘肃兰州730000

年份：2013

卷号：44

期号：23

起止页码：3346

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"国家科技支撑计划课题(2011BAI05B02)

语种：中文

中文关键词：当归;补气作用;特征图谱;谱效关系;灰关联度

外文关键词：Angelicae Sinensis Radix ; reinforcing Qi; specific chromatogram; spectrum-effect relationship; gray relation degree

摘要：目的探讨当归补气活性部位HPLC特征图谱与其补气功效的相关性。方法用控食和疲劳的方法建立小鼠气虚模型;比较当归不同提取部位对气虚小鼠单核巨噬细胞的吞噬功能及免疫器官等的影响,筛选当归补气药效部位。用灰色关联统计方法将补气作用较佳的各批当归药材70%乙醇提取部位(醇提物)HPLC特征图谱各共有峰的峰面积与补气药效数据相关联,研究谱效相关性。结果当归醇提物对气虚症状有一定的改善作用;根据关联度大小确定当归醇提物中各成分对补气作用贡献大小的顺序(按特征峰编号)为18>5>7>12>17>4>19>2>20>6>8>3>14>1>16>11>9>13>15>10,其中7、10、11、13、17、18和20号峰分别为阿魏酸、洋川芎内酯Ⅰ、洋川芎内酯H、洋川芎内酯A、藁本内酯、丁烯基苯酞和欧当归内酯A。结论当归醇提物具有较强的补气作用,HPLC特征图谱与补气作用有一定相关性。
Objective To analyze the correlation between HPLC chromatogram and the effect of different extracted fractions from Angelicae Sinensis Radix （ASR） on reinforcing Qi. Methods Qi deficiency model of mice was set up by controlling diet and fatigue. The influences of the different extracted fractions from ASR on phagocytotic ability of mononuclear macrophage and immune organs of mice with the symptoms of Qi deficiency were compared, and the active fraction of ASR was screened. The part which was extracted with 70% ethanol （ethanol extract） from ASR has the certain improvement on the Qi deficiency symptoms. The peak areas of each common peak from HPLC fingerprint were correlated with the pharmacodynamic efficacy, and the grey relation statistics was used to study the spectrum-effect relationship. Results The contribution of various components in ethanol extract with the effect of reinforcing Qi was determined by the spectrum-effect relationship, the order was as follows （characteristic peak number）： 18〉5 〉 7〉 12〉 17〉4〉 19〉2〉20〉6〉8〉3〉 14〉 1 〉 16〉 11 〉9〉 13〉 15〉 10, peak 7, 10, 11, 13, 17, 18, and 20 are ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide, butylidenephthalide, and levistilide A, respectively. Conclusion The ethanol extract of ASR has the strong activity of reinforcing Qi, and there is a certain correlation between HPLC specific chromatogram and the effect.