文献类型：期刊文献

中文题名：蛇葡萄素钠体外协同卡铂抗小鼠肺癌Lewis细胞的作用

英文题名：Cytotoxic effect of ampelopsin sodium combined with carboplatin on mouse Lewis lung cancer cells

作者：缪苗苗[1,2];张艳霞[3,2];吴勇杰[2];覃红[2]

第一作者：缪苗苗

机构：[1]兰州市第二人民医院,兰州730046;[2]兰州大学基础医学院药理学研究所甘肃省新药临床前研究重点实验室,兰州730000;[3]甘肃中医学院,兰州730000

第一机构：兰州市第二人民医院,兰州730046

年份：2015

卷号：31

期号：2

起止页码：20

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;小鼠肺癌Lewis细胞;细胞凋亡

外文关键词：ampelopsin sodium(蛇葡萄素钠);;carboplatin;;mouse Lewis lung cancer cells;;apoptosis

摘要：目的:研究蛇葡萄素钠单用及与卡铂联合用药对小鼠肺癌Lewis细胞的体外抗肿瘤作用,初步探讨蛇葡萄素钠抑瘤作用机制。方法:应用MTT比色法测定蛇葡萄素钠单用及与卡铂联合用药对小鼠肺癌Lewis细胞增殖的抑制作用;使用流式细胞仪检测蛇葡萄素钠单用及与卡铂合用诱导细胞凋亡作用。结果:MTT实验结果表明,蛇葡萄素钠单用在12.5、25、50、100μg/ml浓度范围内对小鼠肺癌Lewis细胞的增殖具有浓度依赖性的抑制作用;6.25~50μg/ml的蛇葡萄素钠与卡铂3.12~25μg/ml联合用药对卡铂抑制Lewis细胞的增殖作用具有协同效应;流式细胞仪结果表明,蛇葡萄素钠25、50、100μg/ml与卡铂25μg/ml联合应用时,凋亡率显著增高。结论:蛇葡萄素钠单用在一定的浓度范围内对Lewis细胞增殖具有浓度依赖性抑制作用,蛇葡萄素钠与卡铂合用可以增强卡铂的活性,有协同抑制作用,蛇葡萄素钠可能是通过诱导凋亡而发挥其抗肿瘤作用。
Objective: To investigate the cytotoxic effect and mechanism of ampelopsin sodium(AMP-Na) on mouse Lewis lung cancer cells by alone or combined with carboplatin.Methods: Use the MTT colorimetric method to investigate cytotoxicity effect of AMP-Na alone or combined with carboplatin to mouse Lewis lung cancer cells; Use flow cytometry(FCM) analysis the cell apoptosis.Results: Experimental study on cytotoxicity in vitro showed that the proliferation of mouse Lewis lung cancer cells was inhibited by AMP-Na alone in a concentration-dependent manner ranging from 12.5 ~ 100 μg/ml; Combined with carboplatin(3.12 ~ 25 μg/ml),AMP-Na 6.25 ~ 50 μg/ml had synergistic inhibitory effect on the proliferation of Lewis cells; FCM results showed that,the apoptotic ratio in those treated with carboplatin(25 μg/ml)combined with AMP-Na(25,50 and 100 μg/ml) was significant higher than carboplatin group.Conclusion: AMP-Na had depressant effect to the generation of Lewis cell,There is a synergistic cytotoxic effect on Lewis cells treated with AMP-Na combined with carboplatin.The mechanism of AMP-Na is probably that it can induce Lewis cells apoptosis.