文献类型：期刊文献

中文题名：香砂六君子汤对脾胃虚弱型功能性消化不良大鼠的干预机制研究

英文题名：Mechanism of Xiangsha Liujunzi Tang intervention for functional dyspepsia rats with spleen and stomach weakness through the CaM/MLCK/MLC20 pathway

作者：刘梦雅[1,2];成映霞[3,4];白敏[1,2];赵琳娜[1,2];李润法[1,2];张延英[1,2];安耀荣[1];段永强[3,4]

第一作者：刘梦雅

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省实验动物行业技术中心,兰州730000;[3]宁夏区域高发病中医药防治教育部重点实验室,银川750004;[4]宁夏区域高发病中西医结合研究重点实验室,银川750004

第一机构：甘肃中医药大学

年份：2023

卷号：31

期号：2

起止页码：232

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：宁夏回族自治区科技厅重点研发引才专项(2022BSB03080);2022年甘肃中医药大学研究生创新创业基金资助项目(2022CX30,2022CX06)。

语种：中文

中文关键词：香砂六君子汤;脾胃虚弱型;CaM/MLCK/MLC20通路;功能性消化不良

外文关键词：Xiangsha Liujunzi Tang;spleen and stomach weakness type;CaM/MLCK/MLC20 pathway;functional dyspepsia

摘要：目的 探讨香砂六君子汤对脾胃虚弱型功能性消化不良(functional dyspepsia, FD)大鼠的干预机制。方法 将60只SPF级雄性SD乳鼠随机分为空白组(n=10)和造模组(n=50),造模组大鼠采用综合造模法(碘乙酰胺灌服+游泳力竭+饥饱失常)复制脾胃虚弱型FD大鼠模型。模型复制成功后将造模组大鼠随机分为模型组、阳性对照组和香砂六君子汤高、中、低剂量组,共5组,每组10只。空白组和模型组大鼠给予10 mL/(kg·d)生理盐水灌胃,阳性对照组给予1.35 mg/(kg·d)莫沙必利药剂灌胃,香砂六君子汤高、中、低剂量组分别给予12、6、3 g/(kg·d)香砂六君子汤汤剂灌胃,持续干预14 d。造模和给药前后观察大鼠一般状况,测定大鼠体重、3 h进食量。给药干预结束后,测定大鼠胃排空率、肠推进率,采用苏木素-伊红染色观察胃组织病理改变,采用生化试剂盒测定大鼠胃组织匀浆液中Mg2+-ATP酶含量,采用蛋白免疫印迹法检测胃组织中CaM、MLCK、p-MLC20蛋白表达水平。结果 与空白组比较,模型组大鼠毛发枯乱、倦懒怠动、行动缓迟,一般状况评分显著降低,体重、3 h进食量、胃排空率及肠推进率均显著下降(P<0.05);胃组织病理未见明显异常;胃组织中Mg2+-ATP酶含量减少,胃组织中CaM、MLCK、p-MLC20蛋白表达水平均显著降低(P<0.05)。与模型组比较,高、中剂量给药组大鼠一般状况评分显著提升,体重、3 h进食量以及胃排空率显著增加(P<0.05);胃组织病理未见明显差异;胃组织中Mg2+-ATP酶含量显著增加,胃组织中CaM、MLCK、p-MLC20蛋白表达水平显著升高(P<0.05),上述指标香砂六君子汤组干预作用呈剂量依赖性。结论 香砂六君子汤能够有效改善脾胃虚弱型FD大鼠一般状况及胃动力,作用机制可能与其激活胃组织中CaM/MLCK/MLC20通路调控平滑肌舒缩,促进胃动力有关。
Objective To explore the mechanisms involved in Xiangsha Liujunzi Tang intervention for functional dyspepsia(FD)in rats with spleen and stomach weakness via the CaM/MLCK/MLC20 pathway.Methods 60 SPF male SD suckling rats were randomly divided into blank(n=10)and modeling(n=50)groups.A comprehensive modeling method(gavage administration of iodoacetamide+exhaustion of swimming+disturbance of hunger and satiety)was used to replicate an FD rat model with spleen and stomach weakness.After successful replication of the model,the rats in the modeling group were randomly divided into a model;positive control;and Xiangsha Liujunzi Tang high?,middle?,and low?dose groups,with 10 rats in each group.Rats in the blank and model groups were given 10 mL/(kg·d)normal saline;the positive control group was given 1.35 mg/(kg·d)mosapride;and the high?,middle?,and low?dose Xiangsha Liujunzi Tang groups were given 12,6,and 3 g/(kg·d)Xiangsha Liujunzi Tang,respectively.The intervention lasted 14 days.The general condition of each rat was observed before and after modeling and administration,and food intake and body mass were measured.After the intervention,the gastric emptying rate and intestinal propulsion rate were measured,and pathological changes to gastric tissue were observed by hematoxylin?eosin staining.The content of Mg2+?ATP enzyme in the gastric tissue was determined biochemically,and the protein expression levels of CaM,MLCK,and p?MLC20 in gastric tissue were detected by Western Blot.Results Compared with the blank group,the model group rats had withered hair;lazy movements;slow actions;significantly reduced general survival score;and lower food intake,body mass,gastric emptying rate,and intestinal propulsion rate(P<0.05),but there was no obvious abnormality in their gastric histopathology.The content of Mg2+?ATP enzyme in the gastric tissue decreased,and the protein expression levels of CaM,MLCK,and p?MLC20 in the gastric tissue decreased significantly.Compared with the model group,the high?and middle?dose group rats had significantly improved general survival status scores,while their food intake,body mass,gastric emptying rate,and intestinal propulsion rate were significantly increased(P<0.05),but there was no significant difference in gastric pathology.The content of Mg2+?ATP enzyme and the protein expression levels of CaM,MLCK,and p?MLC20 in gastric tissue also increased significantly.The intervention effect of Xiangsha Liujunzi Tang was dose dependent.Conclusions Xiangsha Liujunzi Tang effectively improved the general condition and gastric motility of FD rats with spleen and stomach weakness.The specific mechanism may be related to the activation of the CaM/MLCK/MLC20 pathway in gastric tissue to regulate smooth muscle contraction and promote gastric motility.