文献类型：期刊文献

英文题名：Hypoglycemic TCM formulas (Huangqi-Gegen drug pair) have the potential as an Alzheimer's disease

作者：Hai, Yang[1,2,5];Ren, Ke[3];Hou, Wen-qian[3];Cao, Hao-shi[3];Zhang, Ya-rong[3];Li, Zi-mu[3];Wang, Si-qi[3];Yang, Wen[3];Liu, Dong-ling[3,4,5]

第一作者：杨海

通信作者：Hai, Y[1];Liu, DL[1]

机构：[1]Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Key Lab Dunhuang Med, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, 40 Ding Xi Rd 35, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, 40 Ding Xi Rd 35, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：130

外文期刊名：PHYTOMEDICINE

收录：;Scopus(收录号：2-s2.0-85194325796);WOS:【SCI-EXPANDED(收录号:WOS:001247712400001)】；

基金：This work was supported by the National Natural Science Foundation of China [82260792]; Project of Youth Science and Technology Fund of Gansu Province [21JR11RA148]; The Project of Young Doctor Foundation of Colleges and Universities in Gansu Province [22QB-101].

语种：英文

外文关键词：AD; T2DM; C. elegans; Huangqi; Gegen; PTGS2; AChE

摘要：Background: Alzheimer's disease (AD) is a neurological disorder. There is a considerable unmet medical need among those suffering from it. Hypothesis and purpose: Given the link between type-2 diabetes mellitus (T2DM) and AD, hypoglycemic traditional Chinese medicine formulas (TCMFs) may be a treatment for AD. We investigated the possibility of identifying anti-AD medicines in hypoglycemic TCMFs and presented another option for the screening of AD medications. Study design and methods: Paralysis of the transgenic Caenorhabditis elegans (C. elegans) strain CL4176 (caused by amyloid beta (AO)1-42 aggregates) was used to evaluate the anti-AD effect. The toxicity and neurodegeneration induced by neuronal expression of AO in the transgenic C. elegans strain CL2355 were determined using a 5-hydroxytryptamine (5-HT) assay. The transgenic AO-expressing strain CL 2006 and transgenic tau-expressing strain BR5270 were used to explore the effect of TCMFs on protein expression in C. elegans using ELISAs. Then, network pharmacology was used to determine the mechanism of action. The Traditional Chinese Medicine Inheritance Support System platform was used to investigate prescription patterns, core drugs, and optimum combinations of hypoglycemic TCMFs for AD. Results: Sixteen hypoglycemic TCMFs prolonged the PT50 (half paralysis time) of the CL4176 strain of C. elegans, reduced the percentage of worms paralyzed. The results of network pharmacology showed that prostaglandinendoperoxide synthase 2 (PTGS2) and acetylcholine esterase (AChE) are main targets of hypoglycemic TCMFs. Enriched pathway analysis showed that the cholinergic receptor-related pathway was the core pathway of hypoglycemic TCMFs. According to the "four qi and five flavors" system of TCM theory, the main pharmacological qualities were "cold" and "sweet." Through the analysis by TCMISS, we found that Huangqi-Gegen drug pair as the significant Chinese herbs of hypoglycemic TCMFs. The Huangqi-Gegen pairing had the most robust therapeutic effect when delivered at a 2:1 (v/v) ratio. It reduced the paralysis caused by 5-HT, decreased protein expression of AChE and PTGS2, and reduced AO deposition in the brain of the CL2006 strain of C. elegans.