文献类型：期刊文献

英文题名：Rapid separation and enrichment of emodin and rhein in Sanhuang Xiexin Decoction by molecularly imprinted nanoparticles and evaluation of DPP-4 inhibitory activity

作者：Cao, Chuangchuang[1];Li, Yue;Qi, Jingjing[1];Liu, Dandan[1];Feng, Xinyi[1];Zong, Youqi[1];Wang, Yufei[6];Wu, Xiaoyu[1,2,3,4,5];Wu, Guotai[2,3];Duan, Wenda[1,2,3,4,5]

第一作者：Cao, Chuangchuang

通信作者：Duan, WD[1];Duan, WD[2];Duan, WD[3];Duan, WD[4];Duan, WD[5]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Longyao Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[3]Minist Educ & Gansu Prov, Northwest Collaborat Innovat Ctr Chinese & Tibetan, Lanzhou 730000, Gansu, Peoples R China;[4]Key Lab Chem & Qual Res Chinese Tibetan Med Univ G, Lanzhou 730000, Gansu, Peoples R China;[5]Gansu Engn Lab Qual Standardizat Res & Promot Genu, Lanzhou 730000, Gansu, Peoples R China;[6]Northwest Normal Univ, High Sch, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Longyao Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), Minist Educ & Gansu Prov, Northwest Collaborat Innovat Ctr Chinese & Tibetan, Lanzhou 730000, Gansu, Peoples R China;[4]corresponding author), Key Lab Chem & Qual Res Chinese Tibetan Med Univ G, Lanzhou 730000, Gansu, Peoples R China;[5]corresponding author), Gansu Engn Lab Qual Standardizat Res & Promot Genu, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2026

卷号：1777

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20261620502909);Scopus(收录号：2-s2.0-105035569977);WOS:【SCI-EXPANDED(收录号:WOS:001746845600001)】；

基金：This study was supported by the Gansu Provincial Administration of Traditional Chinese Medicine (Grant No: GZKG-2021-53) , Gansu Provincial University Teacher Innovation Fund Project (Grant No 2026A-115) and the Open Fund of the Gansu Provincial Key Laboratory of Pharmacodynamics and Toxicology of Traditional Chinese Medicine (Grant No ZDSYS-KJ-2024-004) .

语种：英文

外文关键词：Dual-template molecularly imprinted polymers; Magnetic molecular imprinting; Molecular simulation; Emodin; Rhein

摘要：To achieve efficient separation of emodin and rhein, a double-template Fe3O4-imprinted polymer (DT-MIP@Fe3O4) was successfully synthesized. The characterization (FT-IR, XRD, TGA, SEM, TEM, VSM) demonstrated that the synthesized DT-MIP@Fe3O4 exhibited a core-shell architecture, exhibiting thermal stability up to 270 degrees C and superparamagnetic characteristics, facilitating separation in 8 s. This material exhibited significant adsorption capacities (31.7823 mg/g and 15.8942 mg/g) and quick adsorption kinetics (equilibrium achieved within 35 min), coupled with the benefits of facile separation, high selectivity, and exceptional reusability. DT-MIP@Fe3O4 was utilized as an adsorbent for magnetic solid-phase extraction (MSPE) in conjunction with high-performance liquid chromatography (HPLC) for the simultaneous separation and detection of emodin and rhein in the Chinese herbal medicine SXD. The optimization of parameters influencing MSPE-HPLC was conducted, such as adsorbent dosage, type and volume of desorbent, desorption time, and salt effect. The established method demonstrated exceptional linearity (R & sup2; > 0.9999) across the 0.1-100 mg/L, exhibiting high sensitivity and stability. The limits of detection (LOD) and the limits of quantification (LOQ) were established at 0.0092-0.02 mg/L and 0.0307-0.0668 mg/L, respectively. Moreover, the separated fractions demonstrated remarkable hypoglycemic efficacy by DPP-4 inhibition. These findings confirmed the effectiveness of the strategy and indicated that it has considerable prospects in the rapid separation of rhein and emodin from complex Chinese herbal compound, providing a method for the search of natural DPP-4 inhibitors.