文献类型：期刊文献

中文题名：温通针法对血管性痴呆大鼠海马烟碱型乙酰胆碱受体表达的影响

英文题名：Warming-promotion needling improves learning-memory ability by up-regulating expression of hippocampal nicotinic acetylcholine receptor subunit in rats with vascular dementia

作者：杨晓波[1,2];王金海[3];安惠琴[1];刘丁龙[1];关姝明[1];崔道宁[1];舒畅[4]

第一作者：杨晓波

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]中国医科大学航空总医院中医科,北京100021;[3]兰州大学第二医院中医科,兰州730030;[4]甘肃中医药大学科研实验中心,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2019

卷号：44

期号：10

起止页码：709

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：国家自然科学基金项目(No.81360563)

语种：中文

中文关键词：温通针法;血管性痴呆;学习记忆能力;烟碱型乙酰胆碱受体;海马

外文关键词：Warming-promotion needling;Vascular dementia;Learning-memory ability;Nicotinic acetylcholine receptor(nAChR);Hippocampus

摘要：目的:观察温通针法对血管性痴呆(VD)大鼠海马烟碱型乙酰胆碱受体(nAChR)亚单位蛋白及mRNA表达水平的影响,探讨其治疗血管性痴呆的作用机制。方法:SD大鼠按随机数字表法分为正常组、模型组、药物组、温通针法组、平补平泻组和捻转针法组,每组10只。采用反复夹闭双侧颈总动脉-再灌注法复制血管性痴呆大鼠模型。药物组给予尼莫地平溶液灌胃,每日2次,共14 d;温通针法组、平补平泻组和捻转针法组于"大椎""百会""水沟"穴分别施以温通针法、平补平泻法和捻转针法针刺,每次留针30 min,每日1次,共治疗14 d。采用Morris水迷宫法检测各组大鼠的行为学,蛋白免疫印迹法和实时荧光定量PCR法检测各组大鼠海马组织nAChR亚单位α4,α7、β2蛋白及mRNA表达的变化。结果:与正常组比较,模型组大鼠逃避潜伏期明显延长,穿越原平台象限的次数明显减少(P<0.05),海马nAChRα4、α7、β2蛋白表达水平明显降低(P<0.05),nAChRα4、α7 mRNA表达水平明显降低(P<0.05),nAChRβ2 mRNA表达水平未见明显改变(P>0.05)。与模型组比较,温通针法组、平补平泻组和捻转针法组大鼠逃避潜伏期均明显缩短(P<0.05),穿越原平台象限的次数均明显增加(P<0.05),且温通针法组优于平补平泻组、捻转针法组(P<0.05);温通针法组、平补平泻组、捻转针法组大鼠海马nAChRα4、α7、β2蛋白表达水平及nAChRα4、α7mRNA的表达水平明显升高(P<0.05),且温通针法组优于平补平泻组、捻转针法组(P<0.05);各组大鼠nAChRβ2 mRNA的表达水平未见明显改变(P>0.05)。结论:温通针法能改善血管性痴呆大鼠的学习记忆能力,保护神经元细胞,其机制可能与其上调海马nAChR表达有关。
Objective To investigate the mechanism of Warming-promotion needling underlying improvement of vascular dementia(VD)by observing its effect on learning-memory ability and expression of hippocampal nicotinic acetylcholine receptor(nAChR)subunit proteins and mRNAs in rats with VD.Methods Sixty male SD rats were randomized into normal control,model,medication,warming-promotion needling(WPN),uniform reinforcing-reducing needling(URRN),and needle-twirling groups(n=10 in each group).The VD model was established via repeated occlusion and reperfusion of the bilateral common carotid arteries.Rats of the medication group were fed with Nimodipine twice a day for 14 days.For rats of the 3 acupuncture groups,WPN,or URRN or needle-twirling was applied to"Dazhui"(GV14),"Baihui"(GV20)and"Shuigou"(GV26)for 30 min,once a day for 14 days.The learning-memory ability was detected by Morris water maze tests,and the expression levels of nAChRα4,α7 andβ2 subunit proteins and mRNAs in the hippocampus were detected by Western blot and real-time PCR,separately.Results Compared with the normal group,the escape latency of Morris water maze tests was significantly prolonged(P<0.05),and the number of the original safe-platform quadrant crossing was significantly reduced in the model group(P<0.05).The expression levels of nAChRα4,α7,β2 subunit proteins and mRNAs(exceptβ2 mRNA)in the hippocampus were significantly decreased in the model group relevant to the normal group(P<0.05).Following the interventions,the increased escape latency,and the reduced safe-platform quadrant crossing times,and the down-regulated expression levels of hippocampal nAChRα4,α7,β2 subunit proteins and mRNAs in the medication,WPN,URRN and needle-twirling groups were all reversed(P<0.05).The effects of WPN were significantly superior to those of URRN and needle-twirling in up-regulating nAChRα4,α7,β2 subunit proteins and mRNAs(P<0.05).Conclusion The warming-promotion needling can improve the learning and memory ability in VD rats,which may be related to its effects in up-regulating the expression of nAChR subunits in the hippocampus.