文献类型：期刊文献

中文题名：基于红外孔板嗅觉测试仪在小鼠嗅觉功能研究中的应用

英文题名：Application of an infrared-based odorized hole-board test instrument in the study of olfactory function in mice

作者：刘彤彤[1];李婧[1,2];董朝玉[1,3];王灿[1,4];解俊樊[1];侯一平[1];邵玉峰[1]

第一作者：刘彤彤

机构：[1]兰州大学基础医学院,甘肃兰州730000;[2]甘肃中医药大学基础医学院,甘肃兰州730000;[3]云南省第三人民医院妇科,云南昆明650011;[4]山东第二医科大学医学影像学院,山东潍坊261053

第一机构：兰州大学基础医学院,甘肃兰州730000

年份：2024

卷号：50

期号：9

起止页码：6

中文期刊名：兰州大学学报（医学版）

外文期刊名：Journal of Lanzhou University（Medical Sciences）

收录：CSTPCD

基金：国家自然科学基金资助项目(31872770,31500853,81771426,82001396)。

语种：中文

中文关键词：嗅觉;嗅觉功能;嗅觉行为学;嗅觉测试仪;红外传感器;小鼠

外文关键词：olfaction;olfactory function;olfactory behavioral experiment;odorized test instrument;infrared sensors;mice

摘要：目的利用灵敏的红外传感器取代电容传感器并用于孔板嗅觉测试仪,建立小鼠嗅觉分辨、识别、空间记忆、偏好实验方法,以检验孔板嗅觉测试仪检测小鼠嗅觉功能的稳定性。方法分别采用习惯/去习惯行为测试、嗅觉空间记忆测试、丙酸嗅觉阈值测试和嗅觉偏好检测正常C57 BL/6J小鼠的嗅觉功能。结果C57 BL/6J小鼠具有嗅觉辨别、嗅觉线索关联的空间记忆能力,对丙酸的嗅觉阈值为10-3kPa,对香叶醇的偏好度最高,对左旋香芹酮的偏好度最低。结论本研究使用的孔板嗅觉测试仪能够满足小鼠嗅觉的辨别、识别、学习记忆以及偏好性等嗅觉行为学实验需求。
Objective To establish experimental methods of olfactory discrimination,recognition,spatial memory and preference in mice by using sensitive infrared sensors instead of capacitive sensors and using them in the odorized hole-board test instrument in order to test the stability of the olfactory functions of mice studied by the instrument.Methods Habituation/dishabituation test,olfactory spatial memory test,propionic acid olfactory threshold test and olfactory preference test were used to evaluate the olfactory function of normal C57 BL/6J mice.Results C57 BL/6J mice were capable of olfactory discrimination and spatial memory of olfactory cue association.The olfactory threshold for propionic acid was 10-3 kPa,and the preference for geraniol the highest,while the preference for L-carvone the lowest.Conclusion The infrared-based odorized hole-board test instrument used in this study can satisfy the requirements of olfactory behavior experiments such as discrimination,recognition,learning and memory,and preference of mice.