文献类型：期刊文献

英文题名：Network Pharmacology Combined with Animal Models to Investigate the Mechanism of ChangPu YuJin Tang in the Treatment of Tourette Syndrome

作者：Lu, Man-Qi[1];Shi, Zheng-Gang[1];Shang, Jing[1];Gao, Lei[1];Gao, Wei-Jiao[1];Gao, Lue[2]

第一作者：Lu, Man-Qi

通信作者：Shi, ZG[1]

机构：[1]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Peoples R China;[2]Shanxi Univ Chinese Med, Clin Med Coll 3, Pediat Teaching & Res Dept, Taiyuan 140100, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

外文期刊名：COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001251051300001)】；

基金：This work was supported by grants from the Gansu Science and Technology Plan Project (No. 20YF3FA041), the National Natural Science Foundation of China (No.81960886), the Shanxi Provincial Department of Education Science and Technology Innovation Project (No. 2022L357), and the Shanxi Provincial Administration of Traditional Chinese Medicine Project (No. 2022ZYYC257).

语种：英文

外文关键词：Tourette syndrome; compound prescription of Chinese medicine; Chang Pu Yu Jin Tang; network pharmacology; BDNF signaling pathway

摘要：Background ChangPu YuJin Tang (CPYJT) is a Chinese herbal formula that has been shown to be an effective therapeutic strategy for pediatric patients with Tourette Syndrome (TS). Using an integrated strategy of network pharmacology and animal model, the aim of this study was to investigate the mechanism of CPYJT in the treatment of TS.Methods Compound libraries of CPYJT were established using databases, such as the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The TCMSP database and Swiss Target Prediction database were used to predict the targets. The above results were constructed into a CPYJT-Drug-Component-Target network. Moreover, TS targets were predicted using GeneCards and other databases. The targets corresponding to the potential ingredients in CPYJT and the targets corresponding to TS were taken as the intersections to construct the CPYJT-TS network. The target network was analysed by PPI using the string database. GO and KEGG enrichment analyses were performed on the target network. The whole process was performed using Cytoscape 3.7.2 to make visual network diagrams of the results. CPYJT was characterised by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS). Transmission Electron Microscopy (TEM) was used to observe the structural changes of CPYJT on the neuronal cells of the IDPN model rats. RT-PCR and Western Blot were used to analyse the changes in the mRNA and protein expression levels of BDNF, TrkB, PI3K, and AKT in the cortex, striatum, and thalamus brain regions after CPYJT administration in IDPN model rats.Results Network pharmacology and UHPLC-MS studies revealed that CPYJT acted on the TS through multiple neurotransmitters and the BDNF/TrkB and PI3K/AKT signalling pathways. CPYJT ameliorated neurocellular structural damage in the cortex, striatum, and thalamus of TS model rats. Additionally, CPYJT up-regulated the levels of BDNF, TrkB, PI3k, and AKT in the cortex, striatum, and thalamus of TS model rats.Conclusion It was found that CPYJT protected neuronal cells from structural damage in multiple brain regions and affected the expression levels of BDNF, TrkB, PI3K, and Akt in the cortex, striatum, and thalamus during TS treatment.