文献类型：期刊文献

中文题名：当归补血汤含药血清对血管紧张素Ⅱ诱导肥大心肌细胞能量代谢的影响

英文题名：Effect of Serum Containing Danggui Buxue Decoction on Energy Metabolism of Myocardial Hypertrophy Induced by Angiotensin Ⅱ

作者：徐厚谦[1];孙艳[1];孙樱丹[1]

第一作者：徐厚谦

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：38

期号：5

起止页码：472

中文期刊名：山东中医药大学学报

外文期刊名：Journal of Shandong University of Traditional Chinese Medicine

收录：CSTPCD

基金：甘肃省中医药管理局英才计划(编号:GZK-2010-56)

语种：中文

中文关键词：当归补血汤;血管紧张素Ⅱ;肥大心肌细胞;能量代谢;实验研究

外文关键词：Danggui Buxue decoction ; angiotensin Ⅱ ;hypertrophic cardiomyocytes ; energy metabolism

摘要：目的:探讨当归补血汤含药血清对心衰后心肌能量代谢的影响。方法:体外培养心肌细胞,实验分为空白对照组、血管紧张素Ⅱ(AngⅡ)组、AngⅡ+缬沙坦组、AngⅡ+含药血清组;分别用塞唑蓝(MTT)法检测各组心肌细胞总蛋白含量和细胞活力;酶联免疫法检测乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)活性;激光共聚焦显微镜观察线粒体膜电位(MMP)变化。结果:与空白对照组比较,AngⅡ组心肌细胞总蛋白含量、细胞活力、LDH、SDH活性及MMP显著升高(P<0.05);与AngⅡ组比较,AngⅡ+含药血清组心肌细胞总蛋白含量、细胞活力、LDH、SDH活性及MMP显著降低(P<0.05)。结论:当归补血汤含药血清能通过抑制肥大心肌细胞LDH、SDH活性及MMP的病理性升高起到改善心肌能量代谢障碍,保护心肌细胞的作用。
Objective：To explore the effect of serum containing Danggui Buxue decoction on the myocardial energy metabolism after heart failure. Methods：Myocardial cells were cultured in vitro,the cardiomyocytes were divided into 4 groups ：control group,Ang Ⅱgroup,Ang Ⅱ ＋ valsartan group and AngⅡI ＋ medicated serum group. The cardiomyocytes activity and total protein content were measured by MTT method. The ac- tivities of lactate dehydrogenase（LDH）,succinate dehydrogenase（SDH） were detected by ELISA. Laser con- focal scanning microscopy was used to detect the change of MMP. Results：In Ang Ⅱ group,the cardiomyocytes activity,total protein content,activities of SDH and LDH and the MMP change were significantly higher than those of the control group（P〈0.05）. In Ang [[ ＋ medicated serum group,the cardiomyocytes activity,total protein content,activities of SDH and LDH and the MMP change were significantly lower than those of the AngⅡ group（P〈0.05）. Conclusion：Serum containing Danggui Buxue decoction could ameliorate myocardial energy metabolism and prevent hypertrophic cardiomyocytes from injuring,the mechanism may be related to restrain the activity of LDH,SDH and MMP of myocardial hypertrophy.