文献类型：期刊文献

中文题名：基于网络药理学的黄芪治疗消化系统炎症作用机制研究

英文题名：Study on the mechanism of Astragalus membranaceus in the treatment of digestive system inflammation based on network pharmacology

作者：周雯[1];骆亚莉[1,2];马玉[1];周啸天[1];周世琴[1]

第一作者：周雯

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃中医药大学基础医学院病理教研室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2021

卷号：19

期号：9

起止页码：1

中文期刊名：中国处方药

外文期刊名：Journal of China Prescription Drug

基金：国家自然科学基金(81760804);甘肃省自然科学基金(20JR10RA321);2019年甘肃省中医方药挖掘与创新转化重点实验室开放基金(ZYFY-2019-006)。

语种：中文

中文关键词：黄芪;网络药理学;乙肝;溃疡性结肠炎;萎缩性胃炎;消化系统炎症

外文关键词：Astragalus membranaceus;Network pharmacology;Hepatitis B;Ulcerative colitis;Atrophic gastritis;Inflammation of digestive system

摘要：目的基于网络药理学方法探讨黄芪治疗消化系统炎症的分子作用机制。方法通过中药系统药理学分析平台(TCMSP)数据库预测黄芪有效成分及作用靶点;在Genecards数据库预测乙肝、溃疡性结肠炎、萎缩性胃炎相关靶点;采用Cytoscape 3.7.2软件构建黄芪活性成分靶点基因调控图;使用String数据库构建蛋白互作(PPI)网络,通过度值(degree)筛选关键靶点;使用R语言对黄芪治疗消化系统炎症关键靶点进行基因本体(G0)功能注释和京都基因与基因组百科全书(KEGG)通路富集分析,以探讨黄芪治疗消化系统炎症的作用机制。结果黄芪治疗消化系统炎症的主要活性成分有槲皮素、山奈酚、异鼠李素、刺芒柄花素等,有众多关键靶点,如TP53、VEGFA、AKT1、MAPK、TNF、IL-6等,可能通过调节TNF信号通路、IL-17信号通路、FoxO信号通路、MAPK信号通路、糖尿病并发症的晚期糖基化终末产物及其受体(AGE.RAGE)通路、卡波西肉瘤相关疱疹病毒感染、乙型肝炎等通路共同发挥治疗消化系统炎症的作用。结论基于网络药理学探讨了黄芪治疗消化系统炎症多成分、多靶点、多途径的作用特点,为进一步深入揭示黄芪治疗消化系统炎症作用机制研究提供了新的思路和方法。
Objective To explore the molecular mechanism of Astragalus membranaceus in the treatment of digestive system inflammation based on network pharmacology.Methods The effective components and action targets of Astragalus membranaceus were predicted by Systems pharmacology(TCMSP)database,and the related targets of Hepatitis B,ulcerative colitis and Atrophic gastritis were predicted by Genecards database Gene Regulatory Map of active components in Astragalus membranaceus was constructed by Cytoscape3.7.2 software,and protein interaction(PPI)network was constructed by String database,and key targets were screened by degree Gene ontology(G0)function annotation and Kegg enrichment analysis were performed using R language to explore the mechanism of Astragalus membranaceus in the treatment of digestive system inflammation.Results The main active components of Astragalus membranaceus in the treatment of digestive system inflammation include quercetin,kaemonol,isorhamnetin,formononetin,etc.,with numerous key targets,such as TP53,VEGFA,AKT1,MAPK,TNF,IL-6,etc.,which may regulate TNF signaling pathway,IL-17 signaling pathway,FOXO signaling pathway,MAPK signaling pathway Advanced glycosylation end products of diabetic complications and their receptors(AGE.RAGE)pathway,such as Kaposi's sarcoma-associated herpes virus infection with hepatitis B,play a role in the treatment of digestive system inflammation.Conclusion Based on the network pharmacology,the characteristics of Astragalus membranaceus in the treatment of digestive system inflammation are discussed,it provides a new idea and method for further study on the mechanism of Astragalus in the treatment of digestive system inflammation.