文献类型：期刊文献

中文题名：当归红芪超滤物对放射性心脏病大鼠的影响

英文题名：Effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on radiation heart disease in rats

作者：高翔[1,2];卢国赐[1];任春贞[1];吕欣芳[1];陈其林[1];吴雪[1];支晓东[1];王春玲[1];李应东[1]

第一作者：高翔

机构：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000;[2]甘肃中医药大学附属医院外周介人科,甘肃兰州730020

第一机构：甘肃中医药大学中西医结合学院

年份：2025

卷号：41

期号：4

起止页码：497

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(82260869);甘肃省自然科学基金资助项目(22JR11RA128);甘肃教育揭榜挂帅基金资助项目(2021jyjbgs-03)。

语种：中文

中文关键词：当归红芪超滤物;放射性心脏病;NOD样受体热蛋白结构域相关蛋白3/半胱氨酸天冬氨酸蛋白酶-1/消皮素D信号通路

外文关键词：Radix Angelica Sinensis and Radix Hedysari ultrafiltration;radiation-induced heart disease;NOD-like receptor protein 3/Caspase-1/Gasdermin D signaling pathway

摘要：目的观察当归红芪超滤物(RAS-RH)对NOD样受体热蛋白结构域相关蛋白3/半胱氨酸天冬氨酸蛋白酶-1/消皮素D(NLRP3/Caspase-1/GSDMD)信号通路及放射性心脏病(RIHD)的干预效应。方法用X射线8 Gy剂量单次全身照射构建模型。将SPF级雄性Wistar大鼠随机分为正常组、模型组(X射线8 Gy)和低剂量实验组(X射线8 Gy+25 mg·kg^(-1)·d^(-1)RAS-RH灌胃)、高剂量实验组(X射线8 Gy+50 mg·kg^(-1)·d^(-1)RAS-RH灌胃),每组各10只,在末次给药后2 h末。用心脏彩超评价大鼠心功能,用酶联免疫吸附试验检测血清中炎症因子白细胞介素(IL)-18、IL-1β和肿瘤坏死因子-α(TNF-α)的表达,用蛋白质印迹法检测大鼠心肌组织NLRP3、Caspase-1、GSDMD、IL-18和IL-1β蛋白相对表达水平。结果低、高剂量实验组和正常组、模型组的左心室射血分数(LVEF)分别为(66.61±1.04)%、(71.89±0.69)%、(81.45±1.34)%和(47.62±1.67)%,血清IL-18水平分别为(171.21±3.92)、(145.60±3.37)、(125.51±2.94)和(201.80±4.05)ng·mL^(-1),IL-1β水平分别为(43.03±0.68)、(41.12±1.01)、(36.12±0.98)和(51.19±0.85)ng·mL^(-1),TNF-α水平分别为(484.96±3.35)、(441.24±3.94)、(381.32±4.16)和(566.51±4.98)ng·mL^(-1),心肌组织NLRP3蛋白相对表达水平分别为0.54±0.02、0.34±0.02、0.20±0.02和0.75±0.03,Caspase-1蛋白相对表达水平分别为0.52±0.02、0.44±0.02、0.36±0.02和0.65±0.03,GSDMD-蛋白N端(GSDMD-NT)蛋白相对表达水平分别为0.53±0.02、0.42±0.01、0.33±0.02和0.73±0.03,IL-18蛋白相对表达水平分别为0.50±0.02、0.42±0.01、0.13±0.01和0.63±0.02,IL-1β蛋白相对表达水平分别为0.59±0.02、0.42±0.02、0.27±0.02和0.79±0.03。模型组的上述指标与正常组比较,低、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论RAS-RH可能通过调控NLRP3/Caspase-1/GSDMD信号通路对RIHD具有改善作用。
Objective To observe the intervention effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration(RAS-RH)on the NOD-like receptor thermal protein domain associated protein 3/cysteinyl aspartate specific proteinase-1/gasdermin D(NLRP3/Caspase-1/GSDMD)signaling pathway and radiation-induced heart disease(RIHD).Methods A model was constructed using a single whole-body X-ray irradiation at a dose of 8 Gy.SPF-grade male Wistar rats were randomly divided into the normal group,the model group(X-ray 8 Gy),the experimental-L group(X-ray 8 Gy+25 mg·kg^(-1)·d^(-1)RAS-RH by gavage)and the experimental-H group(X-ray 8 Gy+50 mg·kg^(-1)·d^(-1)RAS-RH by gavage),with ten rats in each group.2 h after the last administration,cardiac function was evaluated using echocardiography;serum levels of inflammatory factors,including interleukin-18(IL-18),IL-1βand tumor necrosis factor-alpha(TNF-α)were measured using enzyme-linked immunosorbent;relative protein expression levels of NLRP3,Caspase-1,GSDMD,IL-18 and IL-1βin myocardial tissue were detected using Western blotting.Results The left ventricular ejection fraction(LVEF)in experimental-L group,experimental-H group,normal group and model group were(66.61±1.04)%,(71.89±0.69)%,(81.45±1.34)%and(47.62±1.67)%;the serum levels of IL-18 were(171.21±3.92),(145.60±3.37),(125.51±2.94)and(201.80±4.05)ng·mL^(-1);the IL-1βlevels were(43.03±0.68),(41.12±1.01),(36.12±0.98)and(51.19±0.85)ng·mL^(-1);the TNF-αlevels were(484.96±3.35),(441.24±3.94),(381.32±4.16)and(566.51±4.98)ng·mL^(-1);the relative expression levels of NLRP3 protein in myocardial tissue were 0.54±0.02,0.34±0.02,0.20±0.02 and 0.75±0.03;the relative expression levels of Caspase-1protein were 0.52±0.02,0.44±0.02,0.36±0.02 and 0.65±0.03;the relative expression levels of GSDMD-NT protein were 0.53±0.02,0.42±0.01,0.33±0.02 and 0.73±0.03;the relative expression levels of IL-18protein were 0.50±0.02,0.42±0.01,0.13±0.01 and 0.63±0.02;the relative expression levels of IL-1βprotein were 0.59±0.02,0.42±0.02,0.27±0.02 and 0.79±0.03,respectively.The indicators in the model group were significantly different compared to the normal group,and the indicators in the experimental-L and experimental-H groups were significantly different from the model group,with statistical significance(all P<0.05).Conclusion RAS-RH may improve RIHD by regulating the NLRP3/Caspase-1/GSDMD signaling pathway.