文献类型：期刊文献

英文题名：Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer

作者：Niu, Xingdong[1];Ma, Fubin[4];Li, Fangying[1];Wei, Cunchun[1];Zhang, Junrui[1];Gao, Zhenhua[3];Wang, Junhong[2,3];Da, Mingxu[2,5]

第一作者：Niu, Xingdong

通信作者：Wang, JH[1];Da, MX[1];Wang, JH[2];Da, MX[3]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou, Peoples R China;[2]Lanzhou Univ, Clin Med Coll 1, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Peoples Hosp Baiyin 1, Dept Gen Surg, Affiliated Hosp 3, Baiyin, Peoples R China;[4]Ningxia Med Univ, Sch Clin Med, Yinchuan, Peoples R China;[5]Gansu Prov Hosp, Dept Surg Oncol, Donggang West Rd 204, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Tradit Chinese Med, Peoples Hosp Baiyin 1, Dept Gen Surg, Affiliated Hosp 3, Baiyin, Peoples R China;[3]corresponding author), Gansu Prov Hosp, Dept Surg Oncol, Donggang West Rd 204, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：24

期号：1

外文期刊名：BMC CANCER

收录：;Scopus(收录号：2-s2.0-85208097608);WOS:【SCI-EXPANDED(收录号:WOS:001347943000003)】；

基金：This study was supported by grants from the Science and Technology Planning Project of Gansu Province (Grant No. 21CX6FD163), the Second Group of Longyuan Young Talents in Gansu Province (Grant No. 2023-11) and the University teachers innovation fund project (Grant No. 2022B-112)

语种：英文

外文关键词：SYT12; Gastric cancer; EMT; Bioinformatics; Proliferation; Migration

摘要：ObjectiveThis study employs integrated bioinformatics analysis and in vitro cellular experiments to elucidate the role of Synaptotagmin-12 (SYT12) in the progression of gastric cancer.MethodsWe utilized databases and platforms such as Xiantao Academic Tools, UALCAN, Kaplan-Meier plotter analysis, and The Cancer Genome Atlas (TCGA) to extract datasets on SYT12 in gastric cancer. We analyzed the relationship between SYT12 expression and the clinicopathological features, prognosis, diagnosis, and immune infiltration of stomach adenocarcinoma (STAD) patients. Verification was conducted using samples from 31 gastric cancer patients. Additionally, in vitro cellular experiments were performed to determine the role and potential mechanisms of SYT12 in the malignant behavior of gastric cancer cells.ResultsComprehensive bioinformatics analysis indicated that SYT12 is highly expressed in most cancers and is associated with promoter DeoxyriboNucleic Acid (DNA) methylation levels. SYT12 expression correlated with clinicopathological features, immune cell infiltration, immune checkpoint gene expression, and poor prognosis in STAD patients. In vitro experiments suggest that SYT12 may promote the proliferation and migration of gastric cancer cells by inducing epithelial-mesenchymal transition (EMT).ConclusionsThis study highlights the significant role of SYT12 in gastric cancer, suggesting its potential as a new target for early diagnosis, treatment, immunological, and prognostic evaluation in gastric cancer, offering new insights for precision medicine in this disease.