文献类型：期刊文献

中文题名：反相高效液相色谱法测定湿生扁蕾中木犀草素和1,7-二羟基-3,8-二甲氧酮的含量

英文题名：Determination of Luteolin and 1,7-dihydroxy-3,8-dimethoxyxanthone in Herbal Gentianopsis paludosa by RP-HPLC

作者：景明[1];李季文[1];樊秦[1];师霞[1]

第一作者：景明

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2008

卷号：15

期号：3

起止页码：43

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD2011_2012，CSCD_E2011_2012】；

基金：甘肃省中医药科研项目(2007050)

语种：中文

中文关键词：湿生扁蕾;1;7-二羟基-3;8-二甲氧基[口山]酮;木犀草素;反相高效液相色谱法

外文关键词：Gentianopsis paludosa; 1,7-dihydroxy-3,8-dimethoxyxanthone; luteolin; RP-HPLC

摘要：目的建立湿生扁蕾药材中木犀草素和1,7-二羟基-3,8-二甲氧基酮的含量测定方法。方法采用反相高效液相色谱法(RP-HPLC),色谱柱为ZORBAXSB-C18(4.6mm×250mm,5μm);流动相为甲醇(A)-0.4%磷酸水溶液(B)梯度洗脱,0～15min,A∶B=50∶50,15～60min,A∶B=55∶45;检测波长260nm;流速1mL/min;柱温30℃。结果1,7-二羟基-3,8-二甲氧酮和木犀草素分别在0.084～0.84μg和0.184～1.84μg范围内线性关系良好,相关系数分别为0.9994和0.9997;平均回收率分别为96.82%和97.19%,RSD均小于3%。结论该方法简便准确,重现行好,可作为湿生扁蕾药材的质量控制方法。
Objective To establish the determination of luteolin and 1,7-dihydroxy-3,8-dimethoxyxanthone in Herbal Gentianopsis paludosa. Method The RP-HPLC with ZORBAX SB-C18 （250 mm×4.6 mm, 5μm） comlum was used. Mobile phase was methanol （A）-0.4% H3PO4 （B） Gradient elution was used, 0-15 min, A：B=50：50, 15-60 min, A：B=55：45, Detection wavelengh was 260 nm. Flow rate was 1.0 mL/min. Temperature of column was 30℃. Result The linear relationship of 1,7-dihydroxy-3,8-dimethoxyxanthone and luteolin were showed at the range of 0.084-0.84μg and 0.184-1.84μg （r=0.9994 and r=0.9997）. The average recovery rate of 1,7-dihydroxy-3,8-dimethoxyxanthone and luteolin were 96.82% and 97.19%, RSD〈3%. Conclusion The method was accurate, reproducible and can be used to control the quality of Gentianopsis paludosa.