文献类型：期刊文献

中文题名：甘肃产华中五味子对成骨细胞成骨能力的影响

英文题名：Effects of Schisandrae Sphenantherae on Osteogenic Capacity of Osteoblasts

作者：赵文君[1];樊秦[2];孙少伯[2];聂蕾[2]

第一作者：赵文君

机构：[1]中国人民解放军第一医院;[2]甘肃中医学院

第一机构：中国人民解放军第一医院

年份：2009

卷号：16

期号：2

起止页码：32

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃省教育厅项目(0606B-08)

语种：中文

中文关键词：华中五味子;血清药理学;成骨细胞;大鼠

外文关键词：Schisandrae Sphenantherae; serum pharmacology; osteoblast; rat

摘要：目的探讨甘肃产华中五味子对大鼠成骨细胞增殖与分化的影响。方法采用血清药理学方法,对体外培养的大鼠成骨细胞以MTT法进行细胞增殖测定、以PNPP法测定碱性磷酸酶(ALP)活性、以茜素红染色进行矿化结节计数。结果培养48h的10%、15%和培养72h的5%、10%华中五味子75%醇提取物血清组的细胞增殖速度较对照组快(P<0.05或P<0.01);培养48h的10%、15%和培养72h的10%华中五味子75%醇提取物血清组的ALP活性较对照组增加(P<0.05);培养2周的矿化结节计数显示,10%华中五味子75%醇提取物血清组多于对照组(P<0.01)。结论甘肃产华中五味子75%醇提取物对体外大鼠成骨细胞的增殖和分化具有促进作用。
Objective To explore the effect of Schisandrae Sphenantherae on proliferation and differentiation of rat osteoblast in vitro. Methods Using the method of serum pharmacology, osteoblast was isolated from calvaria of newborn SD rats by means of modified sequential collagenase digestion and incubated in RPMI 1640 medium. The cell morphology was observed under a phase contrast inverted microscope. MTT assay, ALP activity, mineral node count were detected to determine the status and activities of proliferation and differentiation. Results In 10%, 15% concentration groups after cultured 48 h and 5%, 10% after cultured 72 h, 75% ethanol extracts of Schisandrae Sphenantherae significantly stimulated the proliferation （P 〈 0.05 or P〈0.01）. In 10%, 15% concentration groups after cultured 48 h and 10% after cultured 72 h, ALP activity was increased （P 〈0.05）. In 10% concentration group, 75% ethanol extracts of Schisandrae Sphenantherae increased the number of mineral nodes after cultured 2 w （P〈0.01）. Conclusion 75% ethanol extracts of Schisandrae Sphenantherae had positive effect on proliferation and differentiation of rat osteoblasts in vitro.