文献类型：期刊文献

英文题名：Caryophyllene oxide exhibits anti-cancer effects in MG-63 human osteosarcoma cells via the inhibition of cell migration, generation of reactive oxygen species and induction of apoptosis

作者：Pan, Zheng[1];Wang, Shuan-Ke[1];Cheng, Xiao-Li[2];Tian, Xia-Wei[1];Wang, Jing[1]

第一作者：Pan, Zheng

通信作者：Wang, SK[1]

机构：[1]Lanzhou Univ, Hosp 2, Orthopaed Key Lab Gansu Prov, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Dept Expt Teaching Ctr, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Hosp 2, Orthopaed Key Lab Gansu Prov, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 2, Orthopaed Key Lab Gansu Prov, Lanzhou, Peoples R China.

年份：2016

卷号：11

期号：4

起止页码：817

外文期刊名：BANGLADESH JOURNAL OF PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-84989878568);WOS:【SCI-EXPANDED(收录号:WOS:000390319200008)】；

基金：Authors acknowledge the financial support of Gansu Provincial Natural Science Foundation of China (Grant No. 2013GS10047).

语种：英文

摘要：The main objective of the present study was to evaluate the antitumor and apoptotic effects of caryophyllene oxide in MG-63 human osteosarcoma cells. Cell viability of these cells was evaluated by MTT assay while as in vitro wound healing assay was used to study the effect of caryophyllene oxide on cell migration. Fluorescence microscopy and transmission electron microscopy were used to study the changes in cell morphology once the cells undergo apoptosis. Caryophyllene oxide significantly led to cytotoxicity in MG-63 cells showing dose-dependent as well as time-dependent effects. Caryophyllene oxide led to an inhibition of wound closure significantly. At caryophyllene oxide doses of 20, 80 and 120 mu M, the percentage of cell migration was shown to be 94.2, 67.1 and 14.8% respectively. With an increase in the caryophyllene oxide dose, the extent of apoptosis also increased characterized by cellular shrinkage, membrane blebbing, chromatin condensation and apoptotic body formation.