文献类型：期刊文献

英文题名：Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells

作者：Li, Hailong[1,2];Xie, Shoupin[3];Liu, Xiaojun[4];Wu, Hongyan[1,2];Lin, Xingyao[1,2];Gu, Jing[1,2];Wang, Huping[1,2];Duan, Yongqiang[1]

第一作者：李海龙;Li, Hailong

通信作者：Duan, YQ[1]

机构：[1]Gansu Tradit Chinese Med Univ, Key Lab TCM Pharmacol & Toxicol Gansu Prov, Lanzhou 730000, Gansu, Peoples R China;[2]New Prod Tradit Chinese Med Engn Lab Gansu Prov, Lanzhou 730000, Gansu, Peoples R China;[3]First Peoples Hosp Lanzhou City, Lanzhou 730050, Gansu, Peoples R China;[4]Lanzhou Univ, Hosp 1, Dept Oncol, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

通信机构：[1]corresponding author), Gansu Tradit Chinese Med Univ, Sch Basic Med Sci, 35 Dingxi Rd, Lanzhou 730000, Gansu, Peoples R China.|[107351d2d02a88e1f325f]甘肃中医药大学基础医学院（敦煌医学研究所）;[10735]甘肃中医药大学;

年份：2014

卷号：32

期号：5

起止页码：2118

外文期刊名：ONCOLOGY REPORTS

收录：;Scopus(收录号：2-s2.0-84946411752);WOS:【SCI-EXPANDED(收录号:WOS:000342714000046)】；

基金：We would like to thank KangChen, Bio-teck (Shanghai, China) for microarray experiments. The present study was supported by the Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province under the Grant Open Plan (no: ZDSYS-KJ-2012-003, ZDSYS-KJ-2013-009).

语种：英文

外文关键词：miRNA; microarray profile; matrine; gastric cancer

摘要：Matrine, a major alkaloid extracted from Sophora flavescens, has been reported to possess antitumor properties in several types of cancers, including gastric cancer. However, its mechanisms of action on gastric cancer remain poorly understood. Dysregulation of microRNAs, a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with cancer. The aim of the present study was to demonstrate that matrine treatment altered miRNA expression in SGC7901 cells. Using miRCURYTM microarray analysis, we identified 128 miRNAs substantially exhibiting >2-fold expression changes in matrine-treated cells relative to their expression levels in untreated cells. RT-qPCR was used to show that the levels of 8 miRNAs whose target genes were clustered in the cell cycle pathway increased, while levels of 14 miRNAs whose target genes were clustered in the MAPK signaling pathway decreased. These results were consistent with those from the miRNA microarray experiment. Bioinformatical analysis revealed that the majority of 57 identified enrichment pathways were highly involved in tumorigenesis. In conclusion, the results demonstrated that matrine induces considerable changes in the miRNA expression profiles of SGC7901 cells, suggesting miRNA microarray combined with RT-qPCR validation and bioinformatical analysis provide a novel and promising approach to identify anticancer targets and the mechanisms of matrine involved.