文献类型：期刊文献

中文题名：四倍体黄芩D20急性和亚急性毒性试验研究

英文题名：Study on the acute and sub-acute toxicity of tetraploid Scutellariae Radix

作者：王丽娟[1,2,3];刘玉[1,3];柴俊雯[1,3];杜弢[1,2,3];胡江海[1];朱荣艳[1]

第一作者：王丽娟

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药药理与毒理重点实验室,甘肃兰州730000;[3]甘肃中医药大学药用植物遗传育种研究所,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2017

卷号：36

期号：3

起止页码：11

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：中央本级重大增减支项目(名贵中药资源可持续利用能力建设;2060302);甘肃省中药材产业科技攻关项目(GYC12-05)

语种：中文

中文关键词：黄芩;二倍体;四倍体;急性毒性;亚急性毒性

外文关键词：Scutellariae Radix; tetrapluid; diploid; acute toxicity; sub-acute toxicity

摘要：目的:观察四倍体黄芩D20急性毒性及亚急性毒性,并与二倍体黄芩进行比较,为其临床应用提供初步安全性评价。方法:采用改良寇氏法测定黄芩对小鼠的半数致死量(LD_(50)),若毒性较低,则测定小鼠对黄芩的最大耐受量(MTD)。结果:通过急性毒性预实验未能测出四倍体黄芩水提液LD_(50),而小鼠对其最大耐受量为120 g/kg,相当于临床成人用量的1 200倍。大鼠亚急性毒性试验血液生化指标及其他各项指标未见异常,无明显病理变化。结论:四倍体黄芩同二倍体黄芩相似,均具有较好的安全性,临床用药安全可靠。
Objective：To observe the acute toxicity, tO mice and sub-acute toxicity to rats of tetraploid and diploid ScuteUariae Radix, so as to provide preliminary safety basis for clinical medication, MethOds： According to the modified Karber＇s method, the LD50 of tetraploid and diploid ScuteUarioe Radix waier extraction If the toxicity was low, it was chanced to determine the maximum tolerance （MTD）. In addition, 30--day feeding experiment in rats was carried out for observing sub-acute toxicity of tetraploid and diploid Scutellariae Radix. Results： The preliminary experiment of acute toxicity was failed to detect LD50 The maximum tolerance of tetraploid and diploid Scutellariae Radix to adult mice was 120 g/kg, equivalent to 1200 times of clinical dosage to adult. 30--day feeding experiment showed that blood biochemical indicators were all in the normal value range and the histopathological changes were not observed. Conclusion： Tetrapioid and diploid Scutellariae Radix are relatively safe.