文献类型：期刊文献

中文题名：小秦艽和秦艽核型的比较研究

英文题名：Comparative Studies on Karyotypes of Gentiana dahurica Fisch and G.macrophylla Pall

作者：刘丽莎[1];王岚[1];孙少伯[1];吴迪[1]

第一作者：刘丽莎

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2009

期号：5

起止页码：331

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家中医药管理局项目(04-C5ZL23);甘肃省自然科学基金项目(3ZS041-A25-066)

语种：中文

中文关键词：小秦艽;秦艽;核型

外文关键词：Gentiana dahurica Fisch; Gentiana macrophylla Pall; karyotype

摘要：目的首次对小秦艽和秦艽的核型进行比较研究,为其遗传和进化提供了进一步的细胞学证据。方法根尖用0.002mol·L-1的8-羟基喹啉溶液预处理5.7h,用2.5%混合酶液,于25℃下酶解1.6h,低渗处理3h(置于冰箱内),然后用空气干燥法制片。结果小秦艽的核型公式是K(2n)=26=2M+24m,属于"1A"型,染色体相对长度组成为2n=26=2L+10M2+12M1+2S;秦艽的核型公式是K(2n)=26=2M+20m+4sm,属于"2B"型,染色体相对长度组成为2n=26=6L+6M2+8M1+6S。结论小秦艽的核型与秦艽相比较,秦艽较为进化。
OBJECTIVE To study karyotypes of Gentiana dahurica Fisch and G. macrophylla Pall for the first time. This will provide cytological evidence for further studies on genetics and evolution. METHODS The root tip was soaked in 0.002 mol-L-1 8-oxychinolin solution for 5.7 h. Then, the sample was decomposed in 2.5% mixed enzyme solution for 1.6 h at 25℃ and treated with Hypotonic for 3 h in refrigerator. At last, specimen slides were prepared by the air-drying technique. RESULTS The karyotypes formula of G. dahurica was K（2n）=2X=26=2M＋24m, which belonged to ＂IA＂ and whose relative length of chromosomes was 2n=26=2L＋10M2＋12M1＋2S. The karyotypes formula of G. macrophylla was K（2n）=2X=26=2M＋20m＋4sm, which belonged to ＂2B＂ and whose relative length of chromosomes was 2n=26=6L＋6M2＋8M1＋6S. CONCLUSION Comparing karyotypes of G. dahurica with G. macrophylla, the result showed that G. macrophylla lives in higher stage of evolution than G. dahurica.