文献类型：期刊文献

英文题名：Proteomics and Metabolomics Unveil Codonopsis pilosula (Franch.) Nannf. Ameliorates Gastric Precancerous Lesions via Regulating Energy Metabolism

作者：He, Rupu[1];Ma, Ruyun[1];Jin, Zheng[1];Zhu, Yanning[1];Yang, Fude[2];Hu, Fangdi[1];Dai, Jianye[1,3]

第一作者：He, Rupu

通信作者：Hu, FD[1];Dai, JY[1];Yang, FD[2];Dai, JY[3]

机构：[1]Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Sch Pharm, Lanzhou, Peoples R China;[3]Lanzhou Univ, Collaborat Innovat Ctr Northwestern Chinese Med, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Sch Pharm, Lanzhou, Peoples R China;[3]corresponding author), Lanzhou Univ, Collaborat Innovat Ctr Northwestern Chinese Med, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000835080400001)】；

语种：英文

外文关键词：proteomics; metabolomics; gastric precancerous lesions; energy metabolism; Codonopsis Radix

摘要：Objective: This study aimed to systematically evaluate the efficacy of Codonopsis pilosula (Franch.) Nannf. (Codonopsis Radix, CR) and reveal the mechanism of its effects on suppressing Gastric Precancerous Lesions.Methods: First, we established the GPL rat model which was induced by N-methyl-N '-nitro-N-nitrosoguanidine, a disordered diet, and 40% ethanol. The CR's anti-Gastric Precancerous Lesions effect was comprehensively evaluated by body weight, pathological section, and serum biochemical indexes. Then, quantitative proteomics and metabolomics were conducted to unveil the disturbed protein-network and pharmacodynamic mechanism. Furthermore, serum pharmacology was employed to confirm that CR's anti-gastritis and anti-cancer phenotype in cell models.Results: In animal models, CR had been shown to control inflammation and ameliorate Gastric Precancerous Lesions. Considering the combination of proteomics and metabolomics, we found that CR could significantly reverse the biological pathways related to energy metabolism which were disturbed by the Gastric Precancerous Lesions model. Furthermore, the results of serum pharmacology indicated that the Codonopsis Radix containing serum could ameliorate gastritis injury and selectively inhibit the proliferation of gastric cancer cells rather than normal cells, which was closely related to ATP production in the above mentioned cells.Conclusion: In summary, CR exerted anti-Gastric Precancerous Lesions effects by ameliorating gastritis injury and selectively inhibiting the proliferation of gastric cancer cells rather than normal cells. Proteomics and metabolomics unveiled that its efficacy was closely related to its regulation of the energy-metabolism pathway. This research not only provided new ideas for exploring the mechanism of complex systems such as Chinese herbals but also benefited the treatment strategy of Gastric Precancerous Lesions via regulating energy metabolism.