文献类型：期刊文献

英文题名：Proteomic Analysis of Cerebrospinal Fluid : Toward the Identification of Biomarkers for Early Central Nervous System Infection

作者：Ding, Yumin[1];Wang, Haiyu[1];Li, Kaixu[1];Zhao, Shujing[1];Li, Dehong[2,3]

第一作者：Ding, Yumin

通信作者：Li, DH[1];Li, DH[2]

机构：[1]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Blood Transfus, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Prov Hosp, Dept Blood Transfus, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Blood Transfus, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Dept Blood Transfus, Lanzhou 730000, Gansu, Peoples R China.

年份：2026

卷号：19

起止页码：1

外文期刊名：INFECTION AND DRUG RESISTANCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001692437400001)】；

基金：Funding Research Foundation for Talented Scholars of Gansu Provincial Hospital (2024KYQDJ-B-11) .

语种：英文

外文关键词：cerebrospinal fluid; proteomics; central nervous system infection; early diagnosis; biomarkers

摘要：Objective: Early diagnosis of central nervous system (CNS) infections remains challenging. The aim of this study was to explore and identify potential biomarkers of CNS infection by comparing cerebrospinal fluid (CSF) proteomic characteristics in patients with tuberculous meningitis (TBM), purulent meningitis (PM), and viral meningitis (VM), as well as in patients without CNS infections. Methods: We conducted the first systematic 4D-DIA comparative proteomic analysis of CSF across TBM (N=6), PM (N=6), VM (N=12), and controls (N=12) using pooled samples. Differential proteins were identified, subjected to GO and KEGG enrichment analyses, and used to construct protein-protein interaction networks for hub protein identification. Results: Proteomic analysis revealed significant differences in CSF protein expression profiles among three types of meningitis. In TBM patients, 53 specific proteins were identified, primarily involved in the complement and coagulation cascade as well as the COVID-19-related immune pathway. PPI network analysis highlighted FGA, FGB, C4BPA, and ADAMTS13 as hub proteins exhibiting notably significant expression levels in TBM patients. In PM patients, 9 proteins were identified, predominantly linked to phagosomes and B-cell receptor signaling, with COLEC11 and ESM1 showing significantly higher expression. In VM patients, 29 proteins were identified, mainly associated with complement and coagulation pathways, featuring higher levels of TMED7, MMP17, and CD81. Conclusion: This study delineated the CSF protein profiles in patients with TBM, PM, VM, and non-CNS infections. We identified several potential biomarkers, including FGA, FGB, C4BPA, ADAMTS13, COLEC11, ESM1, TMED7, MMP17, and CD81. Additionally, we proposed hypothetical molecular mechanisms that may offer valuable diagnostic and therapeutic insights for CNS infections. Nonetheless, given that this is a preliminary exploratory study, further validation through multicenter, large-sample cohort studies and functional experiments is necessary to substantiate the clinical relevance and utility of these biomarkers prior to any potential application in clinical practice.