文献类型：期刊文献

中文题名：鳖甲育肝颗粒对肝纤维化大鼠的作用机制研究

英文题名：Effect and mechanism of Biejia Yugan Granules on rats with liver fibrosis

作者：丁茂鹏[1];韦凌霞[1];王志旺[1];庞亚蓉[1];赵余珠[1]

第一作者：丁茂鹏

机构：[1]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：37

期号：10

起止页码：1167

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金资助项目(81860787);甘肃省高等学校科研基金资助项目(2015A-096)。

语种：中文

中文关键词：鳖甲育肝颗粒;肝纤维化;阴虚证;结缔组织生长因子;秋水仙碱

外文关键词：Biejia Yugan Granules;liver fibrosis;Yin deficiency syndrome;connective tissue growth factor;colchicine

摘要：目的研究鳖甲育肝颗粒对肝纤维化大鼠阴虚证及结缔组织生长因子(CTGF)表达的影响。方法采用皮下注射四氯化碳同时灌胃乙醇的方法来复制肝纤维化大鼠模型,连续42 d。按照体重将大鼠随机分为6组:正常组、模型组、对照组和低、中、高3个剂量实验组,每组8只。从造模开始,实验组灌胃给予鳖甲育肝颗粒1.85,3.70,7.40 g·kg^(-1),对照组给予秋水仙碱0.1 mg·kg^(-1),正常组和模型组给予纯净水,1次/天,连续给药6周。以大鼠舌面湿度等指标评价大鼠阴虚证表征情况。用蛋白质印迹法和实时定量-PCR技术测定CTGF蛋白的相对表达量和CTGF(2-ΔΔCt值)基因表达水平。结果正常组、模型组、对照组和低、中、高3个剂量实验组的舌面湿度分别为(1.01±0.22),(0.69±0.12),(0.80±0.17),(0.88±0.17),(0.94±0.18)和(0.96±0.19)mg;这6组的肝纤维评分分别为(1.19±0.53),(4.81±1.25),(2.44±0.62),(2.88±0.92),(2.56±0.68)和(2.25±0.65)分;这6组的CTGF蛋白的相对表达量分别为0.84±0.12,1.40±0.17,0.91±0.10,1.17±0.14,0.92±0.13和0.83±0.07。上述指标:模型组与正常组比较,差异均有统计学意义(均P<0.05);实验组与模型组比较,差异均有统计学意义(均P<0.05)。基因结果的趋势与蛋白一致。结论鳖甲育肝颗粒可明显缓解肝纤维化大鼠阴虚证表征,而抑制CTGF高表达是其抗纤维化的作用机制之一。
Objective To explore the effect of Biejia Yugan Granule(BYG) on Yin deficiency syndrome and connective tissue growth factor(CTGF) expression of liver fibrosis rats. Methods The rat model of liver fibrosis was established by subcutaneous injection of carbon tetrachloride and intragastric administration of ethanol for 42 d. The SD rats were randomly divided into 6 groups according to weight: normal group, model group, control group and experimental-L, experimental-M and experimental-H groups, 8 rats in each group. At the beginning of model building, experimental-L, experimental-M and experimental-H groups were given the corresponding dose of BYG(1.85, 3.70, 7.40 g·kg-1) and control group was given colchicine(0.1 mg·kg-1) by intragastric administration, as well as the normal group and model group were replaced with purified water, 1 times per a day for 6 weeks. The amount of drinking water and other indicators were used to evaluate the characteristics of yin deficiency syndrome. Connective tissue growth factor(CTGF) protein and its gene expression were determined by Western blot and real-time quantitative-PCR. Results The humidity of tongue in the normal group,model group,control group and experimental-L, experimental-M and experimental-H groups were( 1. 01 ± 0. 22),( 0. 69 ± 0. 12),( 0. 80 ± 0. 17),( 0. 88 ± 0. 17),( 0. 94 ± 0. 18) and( 0. 96 ± 0. 19) mg;the liver histopathology scores of these 6 groups were( 1. 19 ± 0. 53),( 4. 81 ± 1. 25),( 2. 44 ± 0. 62),( 2. 88 ± 0. 92),( 2. 56 ± 0. 68) and( 2. 25 ± 0. 65) point;the relative expression of CTGF protein in liver tissues of above mentioned groups were0. 84 ± 0. 12,1. 40 ± 0. 17,0. 91 ± 0. 10,1. 17 ± 0. 14,0. 92 ± 0. 13 and 0. 83 ± 0. 07. Further,compared with the control group,the above indexes in the model group were all significantly different( P < 0. 05);compared with the model group,the above indexes in the experimental group were all significantly different( P < 0. 05). The trend of gene results is consistent with that of protein. Conclusion BYG can significantly alleviate the signs of Yin deficiency in rats with liver fibrosis,and inhibiting the high expression of CTGF is one of its anti-fibrotic mechanisms.