文献类型：期刊文献

中文题名：针刺对单眼剥夺大鼠视皮层突触结构可塑性的调节机制研究

英文题名：Study on the mechanism of acupuncture on regulation of synaptic structural plasticity in visual cortex of rats with monocular deprivation

作者：朱田田[1,2];邢家铭[1,2];严兴科[1,2]

第一作者：朱田田

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]甘肃省中医院针灸科,兰州730050

第一机构：甘肃中医药大学针灸推拿学院

年份：2017

卷号：32

期号：5

起止页码：2319

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(No.81260560;No.81660816)~~

语种：中文

中文关键词：单眼剥夺;敏感期(可塑期);突触结构;体视学;针刺干预

外文关键词：Monocular deprivation(MD);;Sensitive period(Plastic period);;Synaptic structure;;Stereological analysis;;Acupuncture intervention

摘要：目的:揭示和探讨敏感期内针刺对单眼剥夺(MD)大鼠视皮层结构可塑性的调节作用和机制。方法:将60只SD大鼠随机分为5组,每组12只。其中,空白组不予任何处理;模型组和敏感早、中、末期针刺组采用单侧眼睑缝合的方法复制MD模型后,模型组不予任何治疗,各针刺组分别在造模后第3、12和21天开始针刺治疗。每组治疗结束后进行图形视觉诱发电位(P-VEP)和视皮层17区神经元的形态学检测。结果:与空白组比较,模型组大鼠P_(100)波幅值显著降低(P<0.01),潜伏期明显延迟(P<0.01);而各针刺组P_(100)波幅值明显升高(P<0.01,P<0.05),潜伏期明显提前(P<0.01,P<0.05),较模型组均有显著改善;且敏感早期针刺对P-VEP波形的影响大于敏感中期和敏感晚期针刺。模型组大鼠视皮层17区神经元突触结构出现明显病理损伤,表现为突触结构不清晰,突触间隙消失,髓鞘板层结构松解,细胞间出现局灶状溶解区及髓鞘样改变。而各针刺组大鼠损伤的突触结构均有不同程度修复,其中,早期针刺的修复作用优于中期和晚期针刺。模型组大鼠视皮层17区突触活性区长度明显缩短(P<0.01),而各针刺组突触活性区长度均不同程度延长(P<0.01)。结论:单眼剥夺后大鼠视皮层17区神经元出现电生理及突触超微结构异常改变,表明敏感期内视皮层存在结构和功能可塑性变化;针刺干预能显著调节视觉功能的可塑性变化,并且疗效与治疗时机密切相关。
Objective: To reveal and discuss the regulatory effect and mechanism of acupuncture on the plasticity of visual cortex in monocular deprivation(MD) rats during plastic period. Methods: Sixty SD rats were randomly divided into 5 groups with 12 rats in each group. Blank group was not treated; the model of MD amblyopia through suture of eyelid was reproduced for model and acupuncture groups, and the rats in model group were not receiving any treatment. The rats in early stage acupuncture group were treated after 3 days of model being replicated, and the middle stage and late stage acupuncture were treated after 12 and 21 days respectively. The P-VEP and synaptic ultrastructure of the 17 neurons in the visual cortex of the rats were detected after treatment. Results: Compared with the blank group, model group showed significant changesin P-VEP test, which manifested as the prolongation of the latency and the decrease of the amplitude of P_(100)(P<0.01); while the latency of P_(100) was significantly shortened(P<0.01, P<0.05), and the amplitude was significantly regained(P<0.01, P<0.05) in acupuncture groups; The effect of early stage acupuncture on P-VEP waveform was greater than those of middle and late stage acupuncture. Compared with the blank group, the model group showed significant synaptic structural damage, presenting as unclear synaptic structure, disappeared synaptic cleft, decomposed lamellar structure of myelin sheath, some focal dissolving regions and concentric lamellae changes among the cells. Compared with the visual cortex neurons of the model group, those in the acupuncture group of different stages recovered in different degrees, and the recovery of early acupuncture was better than those of middle and late stage acupuncture. Compared with the blank group, the model group showed a significant reduction in the length of active zone(P<0.01), and compared with the model group, the active zone of three acupuncture groups were significantly lengthened(P<0.01). Conclusion: There are abnormal neuronal ultrastructure and synaptic electrophysiology in visual cortex area 17 of MD rats, showed that the sensitive period exists the cortical plasticity of structure and function. Acupuncture intervention can significantly change the plasticity of visual function, and the effect is closely related to the timing of treatment.