文献类型：期刊文献

英文题名：Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils

作者：Liu, Chaoqun[1];Wang, Yinquan[1,2];Jin, Ling[1,2];Wang, Yan[1];Liu, Dongling[1]

第一作者：刘超群

通信作者：Wang, YQ[1];Jin, L[1];Wang, YQ[2];Jin, L[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2024

卷号：15

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001207519400001)】；

基金：The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the earmarked fund for National Modern Agricultural Industry Technology System Grant (CARS-21) of the Ministry of Finance and the Ministry of Agriculture and Rural Affairs of the People's Republic of China, the Strategic Research and Consulting Project of the Chinese Academy of Engineering (GS2021ZDA06), Double First-Class Major Scientific Research Project of the Gansu Provincial Department of Education (GSSYLXM-05), Evaluation of the Toxicity of Rotten Lilies Based on the Elegant Caenorhabditis elegans (2022CX12), and Gansu Province Science and Technology Major Special Project (23ZDFA013-1).

语种：英文

外文关键词：Lilium davidii var. willmottiae (E. H. Wilson) Raffill; bulb rot disease; Fusarium oxysporum; biological characteristics; anti-fungal effect; plant essential oils

摘要：Lanzhou lily (Lilium davidii var. willmottiae) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accordance with Koch's postulates, and the pathomycetes were identified based on their morphological and molecular characteristics. Furthermore, the biological characteristics of the pathogens were investigated, followed by an evaluation of the antifungal effects of three plant essential oils against them. The results showed that two strains of fungi were isolated from Lanzhou lily rot, which were identified as Fusarium oxysporum Schl. and Aspergillus sydowii (Bain. Et sart.). In addition, the pathogenicity of these two strains of fungi was demonstrated that only F. oxysporum induced rot with similar symptoms during the post-harvest storage period. The biological characteristics of F. oxysporum indicated the potato maltose agar and lily dextrose agar were identified as the most suitable media. Sucrose was determined to be the optimal carbon source, while ammonium nitrate was found to be the best nitrogen source for the growth of F. oxysporum. Mycelial growth and sporulation of F. oxysporum occurred at an optimum pH value of 6. Total darkness facilitated mycelial growth and conidial germination. The ideal temperature for growth was found to be 28 degrees C, while relative humidity did not significantly impact mycelial growth; however, a relative humidity of 55% was most favorable for spore production. Among the three essential oils tested, cinnamon essential oil displayed superior antifungal efficacy against F. oxysporum, whereas angelica essential oil and tea tree essential oil also exhibited moderate inhibitory effects against this pathogen. This research provides valuable theoretical insights for disease control during the storage and transportation of Lanzhou lily.