文献类型：期刊文献

中文题名：基于网络药理学的党参增强免疫功能机制研究

英文题名：Network Pharmacology-Based Study on Mechanism of Codonopsis Pilosula Enhancing Immune Function

作者：陈冬梅[1];蒙洁[1];刘佳佳[1];赵翊[2];王晶[1]

第一作者：陈冬梅

机构：[1]甘肃中医药大学临床医学院,甘肃兰州730000;[2]甘肃中医药大学公共卫生学院,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2020

卷号：38

期号：2

起止页码：184

中文期刊名：中华中医药学刊

外文期刊名：Chinese Archives of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：国家自然科学基金(81760835);甘肃省高等学校科学研究一般项目(2018A-052)。

语种：中文

中文关键词：党参;免疫功能;网络药理学

外文关键词：Codonopsis pilosula;immune function;network pharmacology

摘要：目的运用网络药理学方法探究党参增强免疫功能的潜在作用机制。方法利用中药系统药理学数据库与分析平台(TCMSP)查找党参的活性成分及与活性成分相关的潜在靶点,并采用Cytoscape3.6.1软件构建党参活性成分-靶点网络。在NCBI网站下Gene数据库中以"immunosuppression"为关键词搜索免疫抑制靶点,使用装载ReactomeFIViz插件的Cytoscape3.6.1软件构建免疫抑制靶点相互作用网络,并将其与党参活性成分-靶点网络融合,获得党参活性成分的免疫作用靶点。使用DAVID数据库对党参免疫作用靶点进行KEGG通路富集分析,以探究党参增强免疫功能的作用机制。结果党参发挥免疫作用的主要靶点有肿瘤坏死因子(TNF),转录因子p65(RELA),白细胞介素10(IL10),白细胞介素6(IL6),白细胞介素2(IL2)等。其中木樨草素,芹菜素,棕榈酸甲酯,棕榈酸,白术内酯III主要作用于TNF;芹菜素,月桂酸,辣椒素,木樨草素,棕榈酸甲酯主要作用于RELA;木樨草素,棕榈酸甲酯,棕榈酸主要作用于IL-10;月桂酸,木樨草素,棕榈酸甲酯主要作用于IL-6;芹菜素,木樨草素主要作用于IL-2。KEGG分析得到与党参免疫作用有关的通路16条,主要涉及T细胞受体信号通路,NOD样受体信号通路等。结论党参中的免疫活性成分可能通过调控TNF、RELA、IL10、IL6等靶点,T细胞受体信号通路,NOD样受体信号通路等多个途径发挥增强机体免疫功能的作用。
Objective To explore the potential mechanism of Codonopsis pilosula enhancing immune function by network pharmacology.Methods Firstly,the active components and potential targets of Codonopsis pilosula were searched through TCMSP database,andCytoscape3.6.1 was used to build a network between components and targets(component-target network,CTN).Secondly,in the Gene database of NCBI website,the keyword"immunosuppression"was used to search for immunosuppressive targets,and Cytoscape3.6.1 loaded with ReactomeFIViz plugin was used to build the interaction network of immunosuppressive targets.To obtain the immune targets of the active components of Codonopsis pilosula,the interaction network of immunosuppressive targets was fused with the CTN.Finally,the DAVID database was used to perform KEGG pathway enrichment analysis in order to explore the immune mechanism of Codonopsis pilosula.Results TNF,RELA,IL10,IL6 and IL2 were the main immune targets of Codonopsis pilosula.Luteolin,apigenin,methyl palmitate,palmitic acid and atractylenolide III mainly acted on TNF.Apigenin,lauric acid,capsaicin,luteolin and methyl palmitate mainly acted on RELA.Luteolin,methyl palmitate and palmitic acid mainly acted on IL10.Lauric acid,luteolin and methyl palmitate mainly acted on IL6.Apigenin and luteolin mainly acted on IL2.There were 16 KEGG pathways,mainly related to T cell receptor signaling pathway,NOD-likereceptor signaling pathway,etc.Conclusion Immune active components in Codonopsis pilosula show an effect on enhancing immune function by regulating TNF,RELA,IL10,IL6,and Tcellreceptor signaling pathway and NOD-likereceptor signaling pathway.