文献类型：期刊文献

中文题名：壳聚糖纳米颗粒对K562细胞增殖的影响

英文题名：Effect of Chitosan Nanoparticles on the Proliferation of K562 Cells

作者：罗慧英[1,2];黄亚红[1];杨林[1]

第一作者：罗慧英

机构：[1]甘肃中医学院,兰州730000;[2]甘肃省中药药理与毒理学省级重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：31

期号：10

起止页码：1175

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省青年科技基金计划(1208RJYA024)

语种：中文

中文关键词：壳聚糖纳米颗粒;K562细胞;增殖;细胞凋亡

外文关键词：chitosan nanoparticles; K562 cells; proliferation; apoptosis

摘要：目的评价壳聚糖纳米颗粒对K562细胞增殖的影响,并探讨相关机制。方法采用MTT法对壳聚糖纳米颗粒的最佳作用时间和作用浓度进行筛选,免疫荧光技术和透射电镜技术考察壳聚糖纳米颗粒对K562细胞形态的影响,流式细胞仪检测壳聚糖纳米颗粒对线粒体膜电位、细胞内ROS含量和Ca2+浓度的影响。结果壳聚糖纳米颗粒(15μg·mL-1)可以明显诱导K562细胞出现凋亡改变,表现为:细胞核变小,出现核聚缩等现象。同时线粒体膜电位崩解,ROS含量增加,Ca2+浓度增加。结论壳聚糖纳米颗粒对K562细胞的增殖有抑制作用,其机制可能与诱导细胞凋亡有关。
OBJECTIVE To evaluate the effect of chitosan nanoparticles on the proliferation of K562 cells, and to explore the related mechanism. METHODS The best time and concentration of chitosan nanoparticles were screened by MTT method. Immunofluorescence and transmission electron microscope were used to study the effect of chitosan nanoparticles on the morphology of K562 cells. FACS was used to detect the effect of chitosan nanoparticles on MMP, intracellular ROS concentration, and Ca^2＋ concentration. RESULTS Chitosan nanoparticles（15 μg·mL^-1） could induce K562 cells apoptosis, such as nuclei smaller, nuclear condensation phenomena, company with the collapse of mitochondrial membrane potential, increased ROS content, and the increased of Ca^2＋ concentration. CONCLUSION Chitosan nanoparticles can inhibit the proliferation of K562 cells, the mechanism may be related to inducing cell apoptosis.