文献类型：期刊文献

英文题名：Dopamine-polyethyleneimine co-deposition cellulose filter paper for alpha-Glucosidase immobilization and enzyme inhibitor screening

作者：Li, Peng[1];Ma, Xiao-Hui[2];Jin, Ling[2];Chen, Juan[1]

第一作者：Li, Peng

通信作者：Chen, J[1];Jin, L[2]

机构：[1]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2021

卷号：1167

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000632527700001)】；

基金：This work was funded by Special Foundation for National Science and Technology Basic Resources of China (2018FY100701), the Key Program of Natural Science Foundation of Gansu Province, China (20JR10RA584), and the Fourth National Survey of Traditional Chinese Medicine Resources, Special Subsidy for Public Health Services of Traditional Chinese Medicine, China (CS [2017]-66).

语种：英文

外文关键词：Cellulose filter paper; Dopamine-polyethyleneimine co-deposition; Immobilized alpha-glucosidase; Capillary electrophoresis; Inhibitor screening

摘要：In this work, cellulose filter paper (CFP), which is inexpensive and commercially available, was used as the carrier, and the immobilized alpha-glucosidase was obtained by two steps: firstly, the surface of CFP was modified by polydopamine/polyethyleneimine (PDA/PEI) co-deposition method to obtain CFP-PDA/PEI with a uniform coating of rich positive charge; subsequently, alpha-glucosidase was immobilized on the CFP-PDA/PEI by electrostatic adsorption. The free enzyme and immobilized enzyme have the same optimal temperature (70 degrees C) and pH (8.0), and their K-m is similar, which is 2.2 and 2.8, respectively. These results show that the immobilization process does not change the properties of the enzyme greatly. The immobilized enzyme still maintains 75.6% of its initial activity after 10 repeated uses, showing good reusability. The excellent repeatability (RSD = 2.2%, n = 5) and the verification of competitive inhibitor (acarbose) illustrates the reliability of the immobilized enzymes for enzyme inhibitor screening. Finally, combined with CE, a screening method based immobilized alpha-glucosidase was proposed and applied to screen the alpha-glucosidase inhibitory from 10 kinds of Traditional Chinese medicines (TCMs) in vitro. The results indicated that the method was a very effective tool for screening potential alpha-glucosidase inhibitors from TCMs.