文献类型：期刊文献

英文题名：A novel ratiometric peptide-based fluorescent probe for sequential detection of Hg²⁺ and S^2- ions and its application in living cells and zebrafish imaging

作者：Zhou, Miao[1];Zheng, Maoyue[1];Wang, Peng[1];An, Yong[2]

第一作者：Zhou, Miao

通信作者：Wang, P[1];An, Y[2]

机构：[1]China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China;[2]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730030, Peoples R China

第一机构：China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China

通信机构：[1]corresponding author), China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730030, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：309

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20240215363873);Scopus(收录号：2-s2.0-85181828799);WOS:【SCI-EXPANDED(收录号:WOS:001153183400001)】；

基金：This work was supported by the National Natural Science Foundation of China (NO. 22206154, 82303568) , the Application Foundation Project of Science and Technology Department of Sichuan Province (NO. 2021YJ0409) .

语种：英文

外文关键词：Peptide-based probe; Hg2+; S2-; Zebrafish imaging; Living cells

摘要：A new ratiometric peptide -based fluorescent probe DWPH was designed and synthesized, comprising dansyl fluorophore as a fluorescent dye, and tripeptide backbone (Trp-Pro-His-NH2) as a recognition group. The addition of Hg2+ caused the maximum emission peak of DWPH to blue shift from 560 nm to 510 nm. DWPH exhibited large Stokes shift (230 nm), satisfactory water solubility (100 % aqueous medium), good selectivity (only Hg2+), high sensitivity (24.6 nM), rapid response (within 50 s) and strong anti -interference ability for Hg(2+ )detection over a wide pH range (7-11). Additionally, the complex DWPH-Hg2+ as a relay response probe could also be applied to S2- according to displacement approach. Notably, the detection limit for S2- was calculated as 23.3 nM, exhibiting that DWPH showed great potential for environmental monitoring and bioimaging. In addition, DWPH were successfully used to determine Hg(2+ )and S2- in living cells and zebrafish based on excellent permeability and low cytotoxicity. What's more, the gradient concentration color changes of Hg2+ and S2- were combined with the smartphone APP to obtain red-green-blue (RGB) values, thus enabling rapid semiquantitative detection of Hg(2+ )and S2- without expensive instruments.