文献类型：期刊文献

中文题名：当归红芪合剂超滤膜提取物对ECV-304细胞血管内皮生长因子mRNA表达的影响

英文题名：Effects of Ultra-filtration Extract from Angelica Sinensis and Hedysarum Polybotrys’s Mixture on VEGF mRNA Expression of ECV-304 Cell

作者：刘凯[1]

第一作者：刘凯

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2010

卷号：17

期号：3

起止页码：36

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃省教育厅科研基金(0606-04)

语种：中文

中文关键词：当归;红芪;内皮细胞;血管内皮生长因子

外文关键词：Angelica sinensis; Hedysarum polybotrys; ECV-304 cell; VEGF

摘要：目的探讨当归红芪合剂超滤膜提取物对人脐静脉内皮细胞(ECV-304)血管内皮生长因子(VEGF)mRNA表达的影响。方法以体外培养的ECV-304细胞为模型,采用四氮唑蓝比色法(MTT法)测定细胞增殖,半定量反转录-聚合酶链反应法检测ECV-304细胞VEGFmRNA表达的变化。结果当归红芪合剂超滤膜提取物对ECV-304细胞有促增殖的作用,与对照组比较差异有统计学意义(P<0.05);当归红芪合剂超滤膜提取物能够诱导VEGFmRNA的表达,其作用与剂量呈正相关。结论当归红芪合剂超滤膜提取物对ECV-304细胞的促增殖作用机制是通过诱导ECV-304细胞VEGFmRNA表达实现的。
Objective To study the effect of the ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture on the VEGF mRNA expression of ECV-304 cell. Method Angelica sinensis and Hedysarum polybotrys’s mixture were refined by ultra-filtration technique. ECV-304 cells were cultured as model, and its proliferation was detected by MTT colorimetry. VEGF mRNA expression was observed by semi-quantitative RT-PCR. Results The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture could markly promote the growth of ECV-304 cells, there was significant difference between experimental and control group （P〈 0.05）. The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture upregulated VEGF mRNA expression with dose-dependent relation. Conclusion The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture can markly promote ECV-304 cell proliferation, which may be related to the expression of VEGF mRNA.