文献类型：期刊文献

英文题名：Efficacy of Sishen Wan (???) on dinitrobenzene sulfonic acid- induced ulcerative colitis and its effect on toll-like receptor 2/interleukin-1 receptor-associated kinase-4/nuclear factor-ΚB signal pathway

作者：Zhang Zhaohua[1];Liu Rong[1];Du Nana[3];Zhu Xiangdong[1,2]

第一作者：张志红

通信作者：Zhu, XD[1]

机构：[1]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou 730000, Peoples R China;[2]Ningxia Med Univ, Coll Chinese Med, Yinchuan 750000, Ningxia, Peoples R China;[3]Gansu Prov Canc Hosp, Dept Thorac Surg, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Basic Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：42

期号：4

起止页码：565

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000829121600001)】；

基金：Supported by the National Natural Science Foundation of China: to Explore the Immune Mechanism of Treating Ulcerative Colitis by Regulating Treg/Th17 Cell Imbalance by Warming the Kidney and Invigorating the Spleen through Intestinal Flora (No. 81960826)

语种：英文

外文关键词：colitis; ulcerative; toll-like receptor 2; interleukin-1 receptor-associated kinases; NF-kappa B; signal transduction; therapeutic uses; Sishen Wan

摘要：OBJECTIVE: To investigate the therapeutic effect of Sishen Wan (?????? ???, SSW) on ulcerative colitis (UC) induced by dinitrobenzene sulfonic acid and its effect on toll-like receptor 2/interleukin-1 receptor-associated kinase-4/nuclear factor-Kappa B (TLR2/IRAK4/NF-Kappa B) sig-naling pathway in colonic tissue. METHODS: In this study, 120 Sprague-Dawley rats were randomly divided into blank and model groups. The experimental UC model in rats was established by subcutaneous injection of hydrocortisone + senna gavage for 21 d + dinitrobenzene sulfonic acid (DNBS)/ ethanol solution enema. The successful model rats were randomly divided into the model group; mesalazine (0.36 g/kg) group; and high-, medium-, and low-dose SSW (24, 12, and 6 g/kg) groups. The model and blank groups were gavaged with equal volumes of distilled water once a day for 21 d. The general condition of the rats was observed, and the body mass, fecal properties, and occult blood were recorded for calculating the disease activity index (DAI) score. The colonic tissue of the rats was collected, and its general morphology and pathological form were noted for obtaining the colonic mucosal injury index (CMDI) score. Hematoxylin-eosin staining was used to view the pathological changes of the colon tissue in each group, apoptosis of the cells was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining, and quantitative real-time polymerase chain reaction was used to measure the expressions of TLR2, myeloid differentiation primary response gene 88 (MyD88), IRAK4, and NF-Kappa B p65 mRNA in the colon tissue. The expressions of TLR2, MyD88, IRAK4, and NF-Kappa B p65 protein were detected using western blotting and immunohistochemistry assay, and the levels of interleukin-1[3 (IL-1[3) and tumor necrosis factor-alpha (TNF-alpha) in the colon tissue were determined using enzyme linked immunosorbent assay. RESULTS: Compared with the blank group, the general condition of the model group was relatively poor. The DAI and CMDI scores of the model group increased significantly (P < 0.01), the glands and intestinal mucosa disappeared partially, and several inflammatory cells infiltrated and gathered in the mucosal layer and base layer of the rats in the model group. Furthermore, the cell apoptosis and expression levels of TLR2, MyD88, IRAK4, and NF-Kappa B p65 mRNA and protein in the colon tissue of rats in the model group increased significantly (P < 0.01). The levels of IL-1[3 and TNF-alpha increased significantly in the colon tissue of rats in the model group (P < 0.01). After treatment with SSW, compared with the model group, the general condition of the UC rats improved. Moreover, the DAI and CMDI scores of the UC rats decreased significantly (P < 0.05), and the pathological changes in the colon tissue of the UC rats tended to be normal. The cell apoptosis and expression levels of TLR2, MyD88, IRAK4, and NF-Kappa B p65 mRNA and protein in the colon tissue of the UC rats decreased gradually (P < 0.01), and the levels of IL-1[3 and TNF-alpha decreased significantly (P < 0.01). CONCLUSION: SSW can improve the general condition and alleviate the intestinal mucosal injury of UC model rats. Additionally, SSW can inhibit the TLR2/IRAK4/ NF-Kappa B signaling pathway, but further studies are required to confirm it. (c) 2022 JTCM. All rights reserved.