文献类型：期刊文献

英文题名：Evaluation of Crocetin as a Protective Agent in High Altitude Hypoxia-Induced Organ Damage

作者：Yang, Jun[1,2];Luo, Kai[1,2];Guo, Ziliang[3];Wang, Renjie[1,2];Qian, Qingyuan[2,3];Ma, Shuhe[1,2];Li, Maoxing[1,2,3,4];Gao, Yue[2,4]

第一作者：Yang, Jun;杨静;杨娇;杨晶

通信作者：Li, MX[1];Li, MX[2];Gao, Y[2];Li, MX[3];Li, MX[4];Gao, Y[4]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Beijing Inst Radiat Med, Dept Pharmaceut Sci, Beijing 100850, Peoples R China;[3]Lanzhou Univ, Coll Pharm, Lanzhou 730000, Peoples R China;[4]Natl Key Lab Kidney Dis, Beijing 100850, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Beijing Inst Radiat Med, Dept Pharmaceut Sci, Beijing 100850, Peoples R China;[3]corresponding author), Lanzhou Univ, Coll Pharm, Lanzhou 730000, Peoples R China;[4]corresponding author), Natl Key Lab Kidney Dis, Beijing 100850, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2024

卷号：17

期号：8

外文期刊名：PHARMACEUTICALS

收录：;Scopus(收录号：2-s2.0-85202458948);WOS:【SCI-EXPANDED(收录号:WOS:001305540800001)】；

基金：Traditional Chinese Medicine Innovation Team and Talent Support Program Project from National Administration of Traditional Chinese Medicine (ZYYCXTDD-202207).

语种：英文

外文关键词：crocetin; anti-hypoxia; antioxidant; anti-inflammatory

摘要：Crocetin is an aglycone of crocin naturally occurring in saffron and has been proved to have antioxidant, anti-inflammatory, and antibacterial activities. In this experiment, the protective effect of crocetin on vital organs in high-altitude hypoxia rats was studied. Crocetin was prepared from gardenia by the alkaline hydrolysis method, and its reducing ability and free radical scavenging ability were tested. The in vitro anti-hypoxia vitality was studied on PC12 cells. The anti-hypoxic survival time of mice was determined in several models. The acute hypoxic injury rat model was established by simulating the hypoxic environment of 8000 m-high altitude for 24 h, and the anti-hypoxia effect of crocetin was evaluated by intraperitoneal injection with the doses of 10, 20, and 40 mg/kg. The water contents of the brain and lung were determined, and the pathological sections in the brain, lung, heart, liver, and kidney were observed by HE staining. The levels of oxidative stress (SOD, CAT, H2O2, GSH, GSH-Px, MDA) and inflammatory factors (IL-1 beta, IL-6, TNF-alpha, VEGF) in rat brain, lung, heart, liver, and kidney tissues were detected by ELISA. The results indicated that crocetin exhibited strong reducing ability and free radical scavenging ability and could improve the activity of PC12 cells under hypoxia. After intraperitoneal injection with crocetin, the survival time of mice was prolonged, and the pathological damage, oxidative stress, and inflammation in rats' tissue were ameliorated. The protective activity of crocetin on vital organs in high-altitude hypoxia rats may be related to reducing oxidative stress and inhibiting inflammatory response.